Protein-Arginine Deiminase Type-4 (PADI4) ELISA Kit for Human

Name: Human PADI4 ELISA Kit; Protein-Arginine Deiminase Type-4; MyBioSource
Brand: MyBioSource
SKU: MBS167419
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS167419

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PADI4 antibody. PADI4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PADI4… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

protein-arginine deiminase type-4 (PADI4), ELISA Kit

Full Product Name

Human protein-arginine deiminase type-4 (PADI4) ELISA Kit

Product Gene Name

PADI4 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.05-20ng/ml

Sensitivity

0.021ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

74,079 Da

3D Structure

ModBase 3D Structure for Q9UM07

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PADI4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PADI4 purchase

MBS167419 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protein-arginine deiminase type-4 (PADI4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PADI4. The ELISA analytical biochemical technique of the MBS167419 kit is based on PADI4 antibody-PADI4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PADI4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PADI4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PADI4. It may not necessarily be applicable to this product.

NCBI GI #

216548487

NCBI GeneID

23569

NCBI Accession #

NP_036519.2

UniProt Primary Accession #

Q9UM07

UniProt Secondary Accession #

Q5VTZ8; Q70SX4; A8K392; B2RBW0

UniProt Related Accession #

Q9UM07

GenBank Nucleotide #

NM_012387.2

NCBI Official Full Name

protein-arginine deiminase type-4

NCBI Official Synonym Full Names

peptidyl arginine deiminase 4

NCBI Official Symbol

PADI4

NCBI Official Synonym Symbols

PAD; PAD4; PDI4; PDI5; PADI5

UniProt Protein Name

Protein-arginine deiminase type-4

UniProt Synonym Protein Names

HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV

Protein Family

Protein-arginine deiminase

UniProt Gene Name

PADI4

UniProt Synonym Gene Names

PAD4; PADI5; PDI5

NCBI Protein Information

protein-arginine deiminase type-4

NCBI Summary for PADI4

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

UniProt Comments for PADI4

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

Research Articles on PADI4

1. PADI4 may be closely associated with hypoxiainduced autophagy, and the inhibition of hypoxiainduced autophagy by PADI4 knockdown may contribute to an increase in the apoptosis of RAFLS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PADI4 elisa kit

Reference Product	PubMed Publications
PTPN22 elisa kit	>33 publications with PADI4 and PTPN22
HDAC1 elisa kit	>1 publications with PADI4 and HDAC1
NPM1 elisa kit	>1 publications with PADI4 and NPM1
TP53 elisa kit	>1 publications with PADI4 and TP53

Pathways associated with PADI4 elisa kit

Products by Pathway	Pathway Diagram
Protein Citrullination Pathway antibodies	Protein Citrullination Pathway Diagram
Protein Citrullination Pathway antibodies	Protein Citrullination Pathway Diagram

Diseases associated with PADI4 elisa kit

Disease Name	Pubmed Publications
Arthritis, Rheumatoid Antibodies	>116 publications with PADI4 and Arthritis, Rheumatoid
Inflammation Antibodies	>15 publications with PADI4 and Inflammation
Necrosis Antibodies	>9 publications with PADI4 and Necrosis
Bone Diseases Antibodies	>6 publications with PADI4 and Bone Diseases
Carcinoma Antibodies	>6 publications with PADI4 and Carcinoma
Disease Models, Animal Antibodies	>5 publications with PADI4 and Disease Models, Animal
Adenocarcinoma Antibodies	>4 publications with PADI4 and Adenocarcinoma
Skin Diseases Antibodies	>4 publications with PADI4 and Skin Diseases
Ovarian Diseases Antibodies	>2 publications with PADI4 and Ovarian Diseases
Lung Neoplasms Antibodies	>2 publications with PADI4 and Lung Neoplasms

Organs/Tissues associated with PADI4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>38 publications with PADI4 and Blood
Lung Antibodies	>4 publications with PADI4 and Lung
Brain Antibodies	>3 publications with PADI4 and Brain
Bone Marrow Antibodies	>3 publications with PADI4 and Bone Marrow