Protein-Arginine Deiminase Type-1 (PADI1) ELISA Kit for Human

Name: Human PADI1 ELISA Kit; Protein-Arginine Deiminase Type-1; MyBioSource
Brand: MyBioSource
SKU: MBS167420
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS167420

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PADI1 antibody. PADI1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PADI1… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

protein-arginine deiminase type-1 (PADI1), ELISA Kit

Full Product Name

Human protein-arginine deiminase type-1 (PADI1) ELISA Kit

Product Gene Name

PADI1 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.1-40ng/ml

Sensitivity

0.049ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

74,666 Da

3D Structure

ModBase 3D Structure for Q9ULC6

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PADI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PADI1 purchase

MBS167420 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protein-arginine deiminase type-1 (PADI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PADI1. The ELISA analytical biochemical technique of the MBS167420 kit is based on PADI1 antibody-PADI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PADI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PADI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PADI1. It may not necessarily be applicable to this product.

NCBI GI #

122056685

NCBI GeneID

29943

NCBI Accession #

NP_037490.2

UniProt Primary Accession #

Q9ULC6

UniProt Secondary Accession #

Q70SX6; A1L4K6

UniProt Related Accession #

Q9ULC6

GenBank Nucleotide #

NM_013358.2

NCBI Official Full Name

protein-arginine deiminase type-1

NCBI Official Synonym Full Names

peptidyl arginine deiminase 1

NCBI Official Symbol

PADI1

NCBI Official Synonym Symbols

PDI; PAD1; PDI1; HPAD10

UniProt Protein Name

Protein-arginine deiminase type-1

UniProt Synonym Protein Names

Peptidylarginine deiminase I; Protein-arginine deiminase type I

Protein Family

Protein-arginine deiminase

UniProt Gene Name

PADI1

UniProt Synonym Gene Names

PAD1; PDI1

NCBI Protein Information

protein-arginine deiminase type-1

NCBI Summary for PADI1

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]

UniProt Comments for PADI1

Catalyzes the deimination of arginine residues of proteins.

Research Articles on PADI1

1. Authors showed that PAD1 interacts with and citrullinates MEK1 thereby disrupting MEK1-catalyzed ERK1/2 phosphorylation, thus leading to the MMP2 overexpression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PADI1 elisa kit

Products by Pathway	Pathway Diagram
Protein Citrullination Pathway antibodies	Protein Citrullination Pathway Diagram
Protein Citrullination Pathway antibodies	Protein Citrullination Pathway Diagram

Diseases associated with PADI1 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>1 publications with PADI1 and Carcinoma

Organs/Tissues associated with PADI1 elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>3 publications with PADI1 and Skin
Mouth Antibodies	>1 publications with PADI1 and Mouth