Rho-Associated Protein Kinase 2 (ROCK2) ELISA Kit for Human

Name: Human ROCK2 ELISA Kit; Rho-Associated Protein Kinase 2; MyBioSource
Brand: MyBioSource
SKU: MBS167513
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS167513

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ROCK2 antibody. ROCK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ROCK2… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Rho-associated protein kinase 2 (ROCK2), ELISA Kit

Full Product Name

Human Rho-associated protein kinase 2 (ROCK2) ELISA Kit

Product Gene Name

ROCK2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.2-60ng/ml

Sensitivity

0.08ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

160,387 Da

3D Structure

ModBase 3D Structure for Q62868

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ROCK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROCK2 purchase

MBS167513 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rho-associated protein kinase 2 (ROCK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROCK2. The ELISA analytical biochemical technique of the MBS167513 kit is based on ROCK2 antibody-ROCK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROCK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROCK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ROCK2. It may not necessarily be applicable to this product.

NCBI GI #

398650620

NCBI GeneID

25537

NCBI Accession #

NP_037154.2

UniProt Primary Accession #

Q62868

UniProt Related Accession #

Q62868

GenBank Nucleotide #

NM_013022.2

NCBI Official Full Name

rho-associated protein kinase 2

NCBI Official Synonym Full Names

Rho-associated coiled-coil containing protein kinase 2

NCBI Official Symbol

Rock2

NCBI Official Synonym Symbols

ROK; ROCK-II

UniProt Protein Name

Rho-associated protein kinase 2

UniProt Synonym Protein Names

Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; RhoA-binding kinase 2; p150 ROK-alpha; ROKalpha; p164 ROCK-2

Protein Family

Rho-associated protein kinase

UniProt Gene Name

Rock2

UniProt Synonym Gene Names

ROCK-II; ROKalpha

NCBI Protein Information

rho-associated protein kinase 2

NCBI Summary for ROCK2

a Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD, Feb 2006]

UniProt Comments for ROCK2

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Research Articles on ROCK2

1. acute hypobaric hypoxia augments ROCK2 protein level and activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ROCK2 elisa kit

Reference Product	PubMed Publications
Rhoa elisa kit	>189 publications with ROCK2 and Rhoa
Ppp1r12a elisa kit	>15 publications with ROCK2 and Ppp1r12a
Limk1 elisa kit	>12 publications with ROCK2 and Limk1
Cdc42 elisa kit	>8 publications with ROCK2 and Cdc42
Limk2 elisa kit	>5 publications with ROCK2 and Limk2
Ppp1r14a elisa kit	>5 publications with ROCK2 and Ppp1r14a
Myl9 elisa kit	>1 publications with ROCK2 and Myl9
Slc9a1 elisa kit	>1 publications with ROCK2 and Slc9a1

Pathways associated with ROCK2 elisa kit

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EPH-Ephrin Signaling Pathway antibodies	EPH-Ephrin Signaling Pathway Diagram
EPHA-mediated Growth Cone Collapse Pathway antibodies	EPHA-mediated Growth Cone Collapse Pathway Diagram
EPHB-mediated Forward Signaling Pathway antibodies	EPHB-mediated Forward Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with ROCK2 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>98 publications with ROCK2 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>64 publications with ROCK2 and Disease Models, Animal
Hypertension Antibodies	>61 publications with ROCK2 and Hypertension
Inflammation Antibodies	>52 publications with ROCK2 and Inflammation
Neoplasm Invasiveness Antibodies	>27 publications with ROCK2 and Neoplasm Invasiveness
Fibrosis Antibodies	>25 publications with ROCK2 and Fibrosis
Liver Diseases Antibodies	>18 publications with ROCK2 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>17 publications with ROCK2 and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>16 publications with ROCK2 and Breast Neoplasms
Necrosis Antibodies	>16 publications with ROCK2 and Necrosis

Organs/Tissues associated with ROCK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>53 publications with ROCK2 and Heart
Brain Antibodies	>53 publications with ROCK2 and Brain
Lung Antibodies	>45 publications with ROCK2 and Lung
Kidney Antibodies	>18 publications with ROCK2 and Kidney
Eye Antibodies	>16 publications with ROCK2 and Eye
Embryonic Tissue Antibodies	>12 publications with ROCK2 and Embryonic Tissue
Prostate Antibodies	>10 publications with ROCK2 and Prostate
Connective Tissue Antibodies	>9 publications with ROCK2 and Connective Tissue
Placenta Antibodies	>8 publications with ROCK2 and Placenta
Colon Antibodies	>5 publications with ROCK2 and Colon