Netrin Receptor DCC (DCC) ELISA Kit for Human

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Netrin receptor DCC (also known as DCC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human DCC antibody. DCC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human DCC Antibody is added and binds to DCC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DCC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human DCC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of DCC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS167518 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Netrin receptor DCC (DCC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DCC. The ELISA analytical biochemical technique of the MBS167518 kit is based on DCC antibody-DCC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DCC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DCC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).