Kappa-Type Opioid Receptor (OPRK1) ELISA Kit for Human

Name: Human OPRK1 ELISA Kit; Kappa-Type Opioid Receptor; MyBioSource
Brand: MyBioSource
SKU: MBS167553
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS167553

This human OPRK1 ELISA kit is a quantitative sandwich immunoassay measuring the kappa-type opioid receptor across serum, plasma, cell culture supernates, ascites, and tissue homogenates, supporting pain and receptor-signaling research. Intended Uses: This… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Kappa-type opioid receptor (OPRK1), ELISA Kit

Full Product Name

Human Kappa-type opioid receptor (OPRK1) ELISA Kit

Product Gene Name

OPRK1 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids

Detection Range

0.05-15ng/ml

Sensitivity

0.019ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

33,319 Da

3D Structure

ModBase 3D Structure for P41145

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of OPRK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OPRK1 purchase

MBS167553 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kappa-type opioid receptor (OPRK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPRK1. The ELISA analytical biochemical technique of the MBS167553 kit is based on OPRK1 antibody-OPRK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPRK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPRK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OPRK1. It may not necessarily be applicable to this product.

NCBI GI #

39725940

NCBI GeneID

4986

NCBI Accession #

NP_000903.2

UniProt Primary Accession #

P41145

UniProt Secondary Accession #

Q499G4; E5RHC9

UniProt Related Accession #

P41145

GenBank Nucleotide #

NM_000912.4

NCBI Official Full Name

kappa-type opioid receptor isoform 1

NCBI Official Synonym Full Names

opioid receptor kappa 1

NCBI Official Symbol

OPRK1

NCBI Official Synonym Symbols

KOR; OPRK; KOR-1; K-OR-1

UniProt Protein Name

Kappa-type opioid receptor

Protein Family

Kappa-type opioid receptor

UniProt Gene Name

OPRK1

UniProt Synonym Gene Names

OPRK; K-OR-1; KOR-1

NCBI Protein Information

kappa-type opioid receptor

NCBI Summary for OPRK1

This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]

UniProt Comments for OPRK1

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

Research Articles on OPRK1

1. The OPRK1 gene variants showed significant association with susceptibility to opioid dependence among Iranians.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OPRK1 elisa kit

Reference Product	PubMed Publications
OPRD1 elisa kit	>17 publications with OPRK1 and OPRD1

Pathways associated with OPRK1 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Peptide GPCRs Pathway antibodies	Peptide GPCRs Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with OPRK1 elisa kit

Disease Name	Pubmed Publications
Substance-Related Disorders Antibodies	>13 publications with OPRK1 and Substance-Related Disorders
Pain Antibodies	>10 publications with OPRK1 and Pain
Opioid-Related Disorders Antibodies	>9 publications with OPRK1 and Opioid-Related Disorders
Heroin Dependence Antibodies	>8 publications with OPRK1 and Heroin Dependence
Nervous System Diseases Antibodies	>4 publications with OPRK1 and Nervous System Diseases
Anxiety Disorders Antibodies	>2 publications with OPRK1 and Anxiety Disorders
Cocaine-Related Disorders Antibodies	>2 publications with OPRK1 and Cocaine-Related Disorders
Inflammation Antibodies	>1 publications with OPRK1 and Inflammation
Drug Toxicity Antibodies	>1 publications with OPRK1 and Drug Toxicity
Vomiting Antibodies	>1 publications with OPRK1 and Vomiting

Organs/Tissues associated with OPRK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>12 publications with OPRK1 and Brain
Prostate Antibodies	>2 publications with OPRK1 and Prostate
Muscle Antibodies	>1 publications with OPRK1 and Muscle