Human 20S-PSM ELISA Kit – Cat# MBS2031921

20s-Proteasome (20S-PSM) ELISA Kit for Human

Name: Human 20S-PSM ELISA Kit; 20s-Proteasome; MyBioSource
Brand: MyBioSource
SKU: MBS2031921
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2031921

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to 20S-PSM. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to 20S-PSM. Next, Avidin… More details

$425 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

20S-Proteasome (20S-PSM), ELISA Kit

Full Product Name

Human 20S-Proteasome (20S-PSM) ELISA Kit

Product Gene Name

20S-PSM elisa kit

Product Synonym Names

CP; 20SPSM; 20S Core Particle

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Tissue Homogenates, Cell Lysates And Other Biological Fluids

Detection Range

78-5,000pg/mL

Sensitivity

32pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of 20S-PSM. No significant cross-reactivity or interference between 20S-PSM and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 20S-PSM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 20S-PSM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

27,433 Da

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of 20S-PSM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 20S-PSM purchase

MBS2031921 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 20S-Proteasome (20S-PSM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 20S-PSM. The ELISA analytical biochemical technique of the MBS2031921 kit is based on 20S-PSM antibody-20S-PSM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 20S-PSM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 20S-PSM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for 20S-PSM. It may not necessarily be applicable to this product.

NCBI GI #

284096649

NCBI GeneID

8863741

NCBI Accession #

EFC50276.1

NCBI Official Full Name

20S proteasome

NCBI Official Symbol

NAEGRDRAFT_77167

UniProt Protein Name

Proteasome subunit beta type

Protein Family

Proteasome

UniProt Gene Name

NAEGRDRAFT_77167

UniProt Entry Name

D2UXF6_NAEGR

NCBI Protein Information

20S proteasome

UniProt Comments for 20S-PSM

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with 20S-PSM elisa kit

Products by Pathway	Pathway Diagram
Proteasome Pathway antibodies	Proteasome Pathway Diagram
Proteasome Pathway antibodies	Proteasome Pathway Diagram
Proteasome, 20S Core Particle Pathway antibodies	Proteasome, 20S Core Particle Pathway Diagram
Proteasome, 20S Core Particle Pathway antibodies	Proteasome, 20S Core Particle Pathway Diagram