Inositol 1, 4, 5, -Trisphosphate (IP3) ELISA Kit for Human

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IP3 and Horseradish Peroxidase (HRP) conjugated IP3. The competitive inhibition reaction is launched between with HRP labeled IP3 and unlabeled IP3 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IP3 in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of IP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS702115 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the inositol 1, 4, 5, -trisphosphate (IP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IP3. The ELISA analytical biochemical technique of the MBS702115 kit is based on IP3 antibody-IP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).