Cytoselect 24-Well Collagen Cell Invasion, Colorimetric Assay Kit

Background: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect™ Collagen Cell Invasion Assay Kit utilizes Bovine Type I Collagen-coated inserts to assay the invasive properties of tumor cells. It contains sufficient reagents for the evaluation of 12 samples.

Principle of the Assay: The CytoSelect™ Collagen Cell Invasion Assay Kit contains polycarbonate membrane inserts (8 um pore size) in a 24-well plate. The upper surface of the insert membrane is coated with a uniform layer of dried Bovine Type I Collagen matrix. This collagen matrix layer serves as a barrier to discriminate invasive cells from non-invasive cells. Invasive cells are able to degrade the collagen matrix layer, and ultimately pass through the pores of the polycarbonate membrane. Finally, the cells are removed from the top of the membrane and the invaded cells are stained and quantified.

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Small volumes of CytoSelect 24-well Collagen Cell Invasion, Colorimetric assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168081 is an assay kit targeting CytoSelect 24-well Collagen Cell Invasion, Colorimetric.