Oxiselect Oxidative DNA Damage (Ap Sites) Assay Kit

Background/Introduction: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA. The OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites) uses an Aldehyde Reactive Probe (ARP) to react specifically with an aldehyde group on the open ring form of AP sites. This allows for the AP sites to be tagged with biotin which is later detected with Streptavidin-Enzyme conjugate. The quantity of AP sites in unknown DNA sample is determined by comparing its absorbance with a standard curve generated from the provided DNA standard containing predetermined AP sites. The kit has a detection sensitivity range of 4 to 40 AP sites per 1 x 105 bp. Each kit provides sufficient reagents to perform up to 50 assays for unknown samples, excluding the standard curve.

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Small volumes of OxiSelect Oxidative DNA Damage (AP sites) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168105 is an assay kit targeting OxiSelect Oxidative DNA Damage (AP sites). Also known as OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites).