Oxiselect Uv-Induced DNA Damage (CPD / 6-4pp Quantitation) ELISA Kit

Principle of the Assay: CPD or 6-4PP-DNA standards or unknown DNA samples are first heat denatured before being adsorbed onto a 96-well DNA high-binding plate. The CPD or 6-4PPs present in the sample or standard are probed with an anti-CPD or anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. The CPD or 6-4PP content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined CPD-DNA or 6-4PP-DNA standards.

Background/Introduction: Absorption of ultraviolet (UV) light produces two predominant types of DNA damage, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP) (Figure 1). The result is a transition of C to T and CC to TT, which are the most frequent mutations of p53 in both human and mouse skin cancers. UV damaged DNA is usually repaired by nucleotide excision repair (NER) or base excision repair (BER). After UV exposure, cells activate p53 and stall the cell cycle for repair. If the damage is too severe, the cell will trigger apoptosis to get rid of DNA damaged, potentially mutant cells. OxiSelect™ Oxidative UV-induced DNA Damage ELISA Combo Kit (CPD / 6-4PP Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of CPD or 6-4PP in any DNA samples. The quantity of CPD or 6-4PP in unknown sample is determined by comparing its absorbance respectively with that of a known CPD-DNA or 6-4PP-DNA standard curve. CPD and 6-4PP are assayed individually in separate wells, and each kit provides sufficient reagents to perform up to a total of 96 assays for CPD and 6-4PP combined, including standard curve and unknown samples. The 96 wells may be divided between CPD and 6-4PP assays at any ratio (e.g. 64 wells for CPD and 32 wells for 6-4PP, or 48 wells for each target).

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Small volumes of OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168106 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation). The ELISA analytical biochemical technique of the MBS168106 kit is based on OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation) antibody-OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OxiSelect UV-Induced DNA Damage (CPD / 6-4PP Quantitation). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).