Cytoselect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit

Background: Proliferating Cell Nuclear Antigen (PCNA) was originally identified as an auxiliary factor for DNA polymerase delta, and has been described as a DNA sliding clamp that acts as a polymerase processivity factor. PCNA aids in processivity through association with various DNA replication associated proteins. In addition to DNA replication, PCNA function is associated with chromatin remodeling, sister-chromatid cohesion and cell cycle control. PCNA is often used as a marker for cell proliferation. In addition to its role in DNA replication and proliferation, PCNA plays a role in DNA repair. PCNA activates the DNA damage tolerance pathway in two ways. First, PCNA promotes translesion synthesis through DNA polymerase zeta (zeta), whereby DNA replication stalled at a damaged site can be essentially bypassed, ultimately leaving a mutation behind for the sake of cell survival. Secondly, the PCNA promotes the "template switch" pathway where the homologous recombination machinery is recruited to bypass DNA damage. PCNA itself is post-translationally modified with ubiquitin which activates one or the other DNA damage tolerance pathway: mono-ubiqutination of lysine 164 activates translesion synthesis, while further polyubiquitination at lysine 63 favors the "template switch" pathway. CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mL PCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

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Small volumes of CytoSelect Proliferating Cell Nuclear Antigen (PCNA) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168132 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CytoSelect Proliferating Cell Nuclear Antigen (PCNA). The ELISA analytical biochemical technique of the MBS168132 kit is based on CytoSelect Proliferating Cell Nuclear Antigen (PCNA) antibody-CytoSelect Proliferating Cell Nuclear Antigen (PCNA) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CytoSelect Proliferating Cell Nuclear Antigen (PCNA) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CytoSelect Proliferating Cell Nuclear Antigen (PCNA). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).