Quicktiter HBV Core Antigen ELISA Kit

Principle of the Assay: An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.!!Background/Introduction: Hepatitis B is an infection of the liver caused by the hepatitis B virus. HBV is transmitted by exposure to infectious blood or body fluids (e.g. saliva, semen, urine); forms of transmission include unprotected sexual activity, blood transfusion, mother-to-infant transmission, or consuming contaminated food/water. The acute illness causes liver inflammation, vomiting and jaundice, while chronic HBV infection often leads to liver cirrhosis and cancer. Roughly one third of the world's population have been infected with hepatitis B virus. 5-10% of adults and 90% of babies who have been infected will have the virus for the rest of their lives. The infection is preventable by vaccination. Identification of chronic hepatitis B virus (HBV) infection has long been based on HBV serology and measurement of hepatocytic enzymes. With the development of therapies for chronic HBV infection, including interferon and lamivudine, quantitative detection of HBV has been used increasingly as the most important marker for monitoring HBV replication activity, disease progression, and assessing antiviral treatment. Several assays for the quantitative measurement of HBV DNA have been developed, such as PCR-based nucleic acid amplification assays. However, these methods tend to be cumbersome and expensive. QuickTiter™ HBV Core Antigen ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the HBV core protein. The kit has detection sensitivity limit of 1 ng /mL HBcAg. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HBV samples.

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Small volumes of QuickTiter HBV Core Antigen elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168135 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the QuickTiter HBV Core Antigen, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing QuickTiter HBV Core Antigen. The ELISA analytical biochemical technique of the MBS168135 kit is based on QuickTiter HBV Core Antigen antibody-QuickTiter HBV Core Antigen antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect QuickTiter HBV Core Antigen antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, QuickTiter HBV Core Antigen. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).