PhosphoBlocker Blocking Reagent – Protein Phosphorylation

• Superior blocking compared to dry milk or serum
• Enhances low level phosphoprotein signal without increasing background
• Preserves phosphoprotein antigens during Western blot procedures
• Easy-to-use premixed dry blend

Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

Background: Protein phosphorylation-dephosphorylation is one of the major signaling mechanisms for modulating the functional properties of proteins involved in gene expression, cell adhesion, cell cycle, cell proliferation, and differentiation. Proteins can be phosphorylated by protein kinases on specific serine, threonine, or tyrosine residues. The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting.

MyBioSource's PhosphoBLOCKER contains a proprietary formulation that provides several advantages over conventional blockers:

Designed specifically for phosphoprotein blotting

Enhances low level phosphoprotein signal without increasing background

Premixed dry blend, easy to use

1. Martinez, P. et al. (2012). 53BP1 Deficiency Combined with Telomere Dysfunction Activates ATR-Dependent DNA Damage Response. J.Cell.Biol. 197:283-300.
2. Kasuboski, J.M. et al. (2011). Zwint-1 is a Novel Aurora B Substrate Required for the Assembly of a Dynein-binding Platform on Kinetochores. Mol. Biol. Cell. 22:3318-3330
3. Song, A. et al. (2010). A C. Elegans eIF4E-Family Member Upregulates Translation at Elevated Temperatures of MRNAs Encoding MSH-5 and Other Meiotic Crossover Proteins. J. Cell Sci. 123:2228-2237.
4. Ishii, K. et al. (2010). Insect Cytokine Paralytic Peptide (PP) Induces Cellular and Humoral Immune Responses in the Silkworm Bombyx mori. J. Biol. Chem. 285:28635-28642.
5. Ramakrishnan, R. et al. (2009). Characterization of Cdk9 T-loop Phosphorylation in Resting and Activated CD4+ T lymphocytes. J. Leukoc. Biol. 86:1345-1350.

Small volumes of PhosphoBlocker Blocking Reagent reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168232 is a reagent targeting PhosphoBlocker Blocking Reagent.