Rac1/Cdc42 Activation Assay Kit

Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rac1 and Cdc42, 21 kDa proteins, belong to the family of Rho GTPases regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Rac1 and Cdc42 regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac1 or Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades. Rac1/Cdc42 Activation Assay utilizes PAK PBD Agarose beads to selectively isolate and pull-down the active form of Rac or Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac or Cdc42 is detected by western blot analysis using an anti-Rac1 or Cdc42 specific monoclonal antibody (see Figure 3, Figure 5 and Assay Principle). Rac1/Cdc42 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rac1 or Cdc42. The kit includes easily identifiable PAK1 PBD Agarose beads (see Figure 1), pink in color, along with Rac1 and Cdc42 Immunoblot Positive Controls for quick Rac1 or Cdc42 identification. Each kit provides sufficient quantities to perform 20 assays for each small GTPase.

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Small volumes of Rac1/Cdc42 Activation assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168431 is an assay kit targeting Rac1/Cdc42 Activation. Also known as Rac1/Cdc42 Activation Assay Combo Kit.