Cytoselect 96-Well Cell Invasion, Fluorometric Assay Kit

Background: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. The CytoSelect™ 96-well Cell Invasion Assay Kit utilizes basement membrane-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye. The CytoSelect™ 96-well Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. The kit contains sufficient reagents for the evaluation of 96 samples.

Principle of the Assay: The CytoSelect™ 96-well Cell Invasion Assay Kit contains polycarbonate membrane inserts (8 um pore size) in a 96-well plate. The upper surface of the insert membrane is coated with a uniform layer of dried basement membrane matrix solution. This basement membrane layer serves as a barrier to discriminate invasive cells from non-invasive cells. Invasive cells are able to degrade the matrix proteins in the layer, and ultimately pass through the pores of the polycarbonate membrane. Finally, the invaded cells are dissociated from the membrane and subsequently detected with CyQuant GR Dye (Invitrogen).

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Small volumes of CytoSelect 96-well Cell Invasion, Fluorometric assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168452 is an assay kit targeting CytoSelect 96-well Cell Invasion, Fluorometric.