Rac1 G15A Agarose Beads (Active Rac-GEF) Accessory Reagent

• Pull down the active form of Rac1-GEF
• Colored for easy visualization

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rac, a 21 kDa protein, belongs to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Rac influences molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. Cycling between the GDP-bound and GTP-bound state is regulated primarily by two distinct families of proteins: guanine nucleotide exchange factors (GEFs) activate Rho proteins by catalyzing the exchange of GDP for GTP, the GTPase activating proteins or GAPs negatively regulate GTPase function by stimulating GTP hydrolysis.

Similar to Ras mutants, constitutively active or dominant negative Rho GTPase mutants have been used to bind to Rho-GAP and effectors or to Rho-GEFs, respectively. A nucleotide-free GTPase has also been shown to form a high affinity binary complex with Rho-GEFs. Rac1 G15A Agarose beads selectively isolate and pull-down the active form of Rac-GEFs from purified samples or endogenous lysates. Subsequently, the precipitated Rac-GEF is detected by western blot analysis using an anti-Rac-GEF antibody.

1. Arthur, W.T., Ellerbroek, S.M., Der, C.J., and Burridge K. (2002) J. Biol. Chem. 277, 42964-42972
2. Garcia-Mata R., Wennerberg, K., Arthur, W.T., Noren, N.K., Ellenbroek, S.M., and Burridge K. (2006) Methods Enzymol. 406, 425-437.
1. Wu, C. Y. et al. (2014). PI3K regulation of Rac1 Is required for KRAS-Induced pancreatic tumorigenesis in mice. Gastroenterology.147:1405-1416.
2. Colacios, C. et al. (2011). The p.Arg63Trp polymorphism controls Vav1 functions and Foxp3 regulatory T cell development. J. Exp. Med. 208:2183-2191.

Small volumes of Rac1 G15A Agarose Beads (Active Rac-GEF) accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168525 is an accessory reagent targeting Rac1 G15A Agarose Beads (Active Rac-GEF).