Apocii ELISA Kit

Background: Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Plasma apolipoproteins can be grouped into two classes: the nonexchangeable apolipoproteins (ApoB-100 and ApoB-48), and the exchangeable apolipoproteins (ApoAI, ApoAII, ApoAIV, ApoCI, ApoCII, ApoCIII, and ApoE). ApoCII has a length of 78 amino acids. The protein encoded by APOC2 gene is secreted in plasma where it is a component of very low density lipoproteins and chylomicrons. This protein activates the enzyme lipoprotein lipase in capillaries, which hydrolyzes triglycerides and thus provides free ratty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, which is characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Human ApoCII ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human ApoCII in plasma, serum or other biological fluid samples. The kit has detection sensitivity limit of 1 ng/mL human ApoCII. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

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Small volumes of ApoCII elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168588 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ApoCII, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ApoCII. The ELISA analytical biochemical technique of the MBS168588 kit is based on ApoCII antibody-ApoCII antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ApoCII antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ApoCII. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).