Oxiselect Protein Carbamylation ELISA Kit

Principle of the Assay: Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 9 (also known as ICE-LAP6, or mch6) is considered to be a caspase in the upstream of apoptotic signal transduction. When mitochondria release cytochrome c, Caspase 9 can form complex with cytochrome c and Apaf1, and be activated at the same time. Activated Caspase 9 can activate Caspase 3 which is the key enzyme of cell apoptosis, thereby promoting subsequent apoptotic signals. Caspase 9 exists in the form of prozyme in the normal state and has no activity. However, Caspase 9 is activated in the apoptotic stage and participates in the apoptotic process. This kit is used to conjugate Caspase 9 sequence-specific peptides acetyl-Leu-Glu-His-Asp p-nitroanilide (AcLEHD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 9, the yellow group pNA is dissociated. pNA has an absorption peak at 405nm. Measure the OD value at 405 nm and then Caspase 9 activity can be calculated accordingly!!Background/Introduction: The Caspase 9 Activity Assay Kit can detect the activity of Caspase 9 in cells or tissue lysis.

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Small volumes of OxiSelect Protein Carbamylation elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168597 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OxiSelect Protein Carbamylation, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OxiSelect Protein Carbamylation. The ELISA analytical biochemical technique of the MBS168597 kit is based on OxiSelect Protein Carbamylation antibody-OxiSelect Protein Carbamylation antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OxiSelect Protein Carbamylation antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OxiSelect Protein Carbamylation. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).