Cgmp ELISA Kit

Principle of the assay: An anti-Rabbit IgG polyclonal coating antibody is adsorbed onto a microtiter plate. Cyclic GMP present in the sample or standard competes with Peroxidase cGMP Tracer for plate binding, in the presence of Rabbit Anti-cGMP Polyclonal Antibody. Following incubation and wash steps, any Peroxidase cGMP Tracer bound to the plate is detected with addition of Substrate Solution. The colored product formed in inversely proportional to the amount of cGMP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from cGMP Standard and sample concentration is then determined.

Background: Guanosine 3',5'-cyclic monophosphate (cGMP) is a multi-functional second messenger involved in various cellular activities in many cell and tissue types. It is converted from guanosine triphosphate (GTP) via guanylyl cyclases (GC). It has been shown that the level of cGMP is typically 10-100 fold lower than cAMP in most tissues. cGMP primarily affects cellular activities through four different pathways. These include cGMP-dependent Protein Kinases (PKG/GK), cyclic nucleotide-gated (CNG) channels, cAMP-dependent Protein Kinase (PKA), and Phosphodiesterases. PKGs (PKG I and PKG II) are serine/threonine kinases activated by cGMP. PKG I has several putative targets, many of which are involved in the regulation of smooth muscle cell (SMC) contractility. Other cGMP/PKG I substrates that affect contractility include the myosin-binding subunit (MBS) of MLC Phosphatase and the small GTPase Rho. In addition to its role in smooth muscle relaxation, cGMP/PKG I may also regulate cell survival, proliferation, axon guidance, synaptic plasticity, inflammation, and angiogenesis. cGMP ELISA Kit is a competitive enzyme immunoassay designed to measure cGMP in cell culture supernatants, plasma, serum, saliva, urine, and cell lysates. The kit selectively measures cGMP levels without any significant cross reactivities to other nucleotides or cyclic nucleotides. Samples containing low cGMP levels may be acetylated (reagents provided) for increased sensitivity. Under non-acetylated conditions, the kit has a detection range of 1 to 1000 pmol/mL cGMP; however, under acetylated conditions, the sensitivity is enhanced (approx 50X) to a detection range of 100-6250 fmol/mL.

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Small volumes of cGMP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168636 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing cGMP. The ELISA analytical biochemical technique of the MBS168636 kit is based on cGMP antibody-cGMP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cGMP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, cGMP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).