GGA3 PBD Agarose Beads Accessory Reagent – Small Gtpase Assay Beads

• Pull down the active form of Arf
• Colored for easy visualization

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Arf, a 20 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include vesicle trafficking, organelle structure, and endocytosis. Like other small GTPases, Arf regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP bound) state, Arf1 and Arf6 bind specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.

1. Moss J., Vaughan M. (1998) J. Biol. Chem. 273:21431-214344.
2. Moss J., Vaughan M. (1995) J. Biol. Chem. 270:12327-12330.
3. Kahn R. A., Gilman A. G. (1986) J. Biol. Chem. 261:7906-7911.
4. Rothman J. E. (1994) Nature 372:55-63.
5. Donaldson J. G., Jackson C. L. (2000) Curr. Opin. Cell Biol. 12:475-482.
6. Donaldson J. G., Klausner R. D. (1994) Curr. Opin. Cell Biol. 6:527-532.
7. Dell'Angelica EC, Puertollano R, Mullins C, et al. (2000) J Cell Biol. 149: 81-94.
8. Takatsu H, Yoshino K, Toda K, Nakayama K. (2002) Biochem J. 365: 369-378.
Davis, C. T. et al. (2014). ARF6 Inhibition Stabilizes the Vasculature and Enhances Survival during Endotoxic Shock. J Immunol. 192:6045-6052.

Small volumes of GGA3 PBD Agarose Beads accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168672 is an accessory reagent targeting GGA3 PBD Agarose Beads.