Rac1 Assay Kit

Background: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rac1, a 21 kDa protein, belongs to the family of Rho GTPases regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Rac1 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac1 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades. Rac1 Activation Assay Kit utilizes PAK PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac1 specific monclonal antibody (see Figure 3 and Assay Principle). Rac1 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rac1. The kit includes easily identifiable PAK1 PBD Agarose beads (see Figure 1), pink in color, and a Rac1 Immunoblot Positive Control for quick Rac1 identification. Each kit provides sufficient quantities to perform 20 assays.

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Small volumes of Rac1 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168692 is an assay kit targeting Rac1. Also known as Rac1 Activation Assay.