Rhoa Assay Kit

Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. RhoA, a 21 kDa protein, regulating a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoA regulates molecular events by cycling between an inactive GDP-bound form and an active GTPbound form. In its active (GTP-bound) state, RhoA binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades. RhoA Activation Assay Kit utilizes Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoA specific monoclonal antibody (see Figure 3 and Assay Principle). RhoA Activation Assay Kit provides a simple and fast tool to monitor the activation of RhoA. The kit includes easily identifiable Rhotekin RBD Agarose beads (see Figure 1), pink in color, and a RhoA Immunoblot Positive Control for quick RhoA identification. Each kit provides sufficient quantities to perform 20 assays.

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Small volumes of RhoA assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169193 is an assay kit targeting RhoA. Also known as RhoA Activation Assay.