Cytoselect 96-Well Laminin Assay Kit

Principle of the assay: The CytoSelect 96-well Laminin Cell Invasion Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 96-well plate. The upper surface of the insert membrane is coated with a uniform layer of dried Murine Laminin I matrix. This laminin matrix layer serves as a barrier to discriminate invasive cells from non-invasive cells. Invasive cells are able to degrade the laminin matrix layer, and ultimately pass through the pores of the polycarbonate membrane. Finally, these invaded cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).

Background: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect 96-well Laminin Cell Invasion Assay Kit utilizes Murine Laminin I-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye. CytoSelect 96-well Laminin Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. It contains sufficient reagents for the evaluation of 96 samples.

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Small volumes of CytoSelect 96-well Laminin assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168737 is an assay kit targeting CytoSelect 96-well Laminin. Also known as CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric.