RFP Active Protein

• Includes 6xHis-tag at C-terminus
• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBS

Red fluorescent protein (DsRed) is a spontaneously fluorescent protein isolated from the Indo-Pacific sea coral, Discosoma striata. It absorbs and emits orange-red light and is well suited for multi-color tagging used in FRET. Since the molecular cloning of RFP cDNA and demonstration of RFP as a functional transgene, RFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. RFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the RFP molecule, many RFP variants, such as TagRFP, TurboRFP, DsRed, tdTomato, mCherry, mKate, mRuby, mBanana, mOrange, mPlum, and mStrawberry, have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rRFP requires no additional substrates or cofactors, rRFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.

1. Shaner, N.C. et al, J. Cell Sci. 120: 4247-4260, 2007.
2. Matz, M.V. et al, Nat. Biotechnol. 17: 969-973, 1999.
3. Campbell, R.E. et al, Proc. Natl. Acad. Sci. USA 99: 7877-7882, 2002.
4. Shaner, N.C. et al, Nat. Biotechnol. 22: 1567-1572, 2004.

Small volumes of RFP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168749 is an active protein targeting RFP. Also known as Recombinant RFP.