Rhotekin RBD Agarose Beads Accessory Reagent – Small Gtpase Assay Beads

• Pull down the active form of Rho
• Colored for easy visualization

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rho, a 21 kDa protein, regulating a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Rho regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rho binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades.

1. Ren X.D. and Schwartz M. A. (2000) Methods Enzymol. 325, 264-72.
1. Sabbatini, M. et al. (2008). Rap1 activation plays a regulatory role in pancreatic amylase secretion. J. Biol. Chem. 283:23884-23894.
2. Sabbatini, M. E. et al. (2010). CCK activates RhoA and Rac1 differentially through G-alpha-13 and G-alpha-q in mouse pancreatic acini. Am. J. Physiol. Cell Physiol. 298:C592-C605.

Small volumes of Rhotekin RBD Agarose Beads accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168765 is an accessory reagent targeting Rhotekin RBD Agarose Beads.