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EGFP Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS168819

• Includes 6xHis-tag at C-terminus• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBSGreen Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its… More details

$470 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EGFP, Recombinant Protein
Full Product Name
Recombinant EGFP
Product Synonym Names
Recombinant EGFP
Preparation and Storage
Store at -80°C; avoid freeze/thaw.
Shelf Life: 1 year from date of receipt under proper storage conditions
Purity
Expressed and purified from E. coli as a recombinant protein, greater than 90% by SDS-PAGE (Figure 1). rEGFP has an approximate MW of 29 kDa, containing a 6xHis-tag at the C-terminus, and an excitation/emission spectra of 488/507 nm. Fluorescence activity was determined by GFP Fluorometric Quantitation Kit (MBS168637).
Quantity
100 ug/vial at 1.0 mg/mL in 1X PBS

Testing Data of EGFP recombinant protein

EGFP Recombinant Protein – Cat# MBS168819

Testing Data #2 of EGFP recombinant protein

The following figures demonstrate typical fluorescence rEGFP as tested in the GFP Fluorometric Quantitation Kit (MBS168637). Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff. One should use the data below for reference only. This data should not be used to interpret actual results.
EGFP Recombinant Protein, Testing Data #2 – Cat# MBS168819

Product References and Citations for EGFP recombinant protein

1. Chalfie M. et al, Science 263: 802-805, 1994.
2. Cormack B.P. et al, Gene 173: 33-38, 1996.
3. Rizzuto R. et al, Curr.Biol. 6:183-188 1996.
1. Caschera, F. & Noireaux, V. (2015). Preparation of amino acid mixtures for cell-free expression systems. Biotechniques. 58:40-43.
2. Caschera, F. & Noireaux, V. (2015). A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system. Metab Eng. 27:29-37.
3. Sokolova, E. et al. (2013). Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate. PNAS. 110: 11692-11697.

Other Notes

Small volumes of EGFP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EGFP purchase

MBS168819 is a recombinant protein targeting EGFP. Also known as Recombinant EGFP.
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