Apociii ELISA Kit

Background: Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Plasma apolipoproteins can be grouped into two classes: the nonexchangeable apolipoproteins (ApoB-100 and ApoB-48), and the exchangeable apolipoproteins (ApoAI, ApoAII, ApoAIV, ApoCI, ApoCII, ApoCIII, and ApoE). ApoCIII is a 79-amino-acid protein synthesized by liver and intestine, which is an essential constituent of circulating particles rich in triacylglycerol, i.e., chylomicrons and VLDLs. ApoCIII inhibits the hydrolysis of TG-rich particles by the lipoprotein lipase and their hepatic uptake mediated by ApoE. Human ApoCIII ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human ApoCIII in plasma, serum or other biological fluid samples. The kit has detection sensitivity limit of 50 pg/mL human ApoCIII. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

1. Segrest J. P., Garber D. W., Brouillette C. G., Harvey S. C., and Anantharamaiah G. M. Adv. Protein Chem. 45: 303-369, 1994.
References
2. Segrest J. P., Jones M. K., De Loof H., Brouillette C. G., Venkatachalapathi Y. V., and Anantharamaiah G. M. J. Lipid Res. 33: 141-166, 1992.
3. Mendivil C. O, Zheng C, Furtado J, Lel J, and Sacks F. M. Arteriosclerosis, Thrombosis and Vascular Biology 30 (2): 239-45, 2009.

Small volumes of ApoCIII elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168878 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ApoCIII, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ApoCIII. The ELISA analytical biochemical technique of the MBS168878 kit is based on ApoCIII antibody-ApoCIII antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ApoCIII antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ApoCIII. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).