Oxidized Protein Immunoblot Control (Carbonyl-BSA) Native Protein

• Provided at 50 ug/mL

Protein oxidation is defined as the covalent modification of a protein induced either directly by reactive oxygen species or indirectly by reaction with secondary by-products of oxidative stress. Oxidative modification of proteins can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions.

There are numerous types of protein oxidative modifications. The most common products of protein oxidation in biological samples are the protein carbonyl derivatives of Pro, Arg, Lys, and Thr. These derivatives are chemically stable and serve as markers of oxidative stress for most types of ROS. Many of the assays involve derivitization of the carbonyl group with dinitrophenylhydrazine (DNPH), which leads to formation of a stable dinitrophenyl hydrazone product, followed by imuunoblooting with anti-DNP antibody.

1. Cadenas, E., Boveris, A., Ragan, CI., and Stoppani, AO. (1977). Archives of Biochemistry & Biophysics 248257.
2. Wakeyama, H., Takeshige, K., Takayanagi, R., and Minakami, S. (1982). Biochem J 593601.
3. Talent, JM., Kong, Y., and Gracy, RW. (1998). Anal.Biochem. 3138.
Biswas, C. et al. (2014). Nuclear Heme Oxygenase-1 (HO-1) Modulates Subcellular Distribution and Activation of Nrf2, Impacting Metabolic and Anti-oxidant Defenses. J Biol Chem. 289:26882-26894.

Small volumes of Oxidized Protein Immunoblot Control (Carbonyl-BSA) native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168896 is a native protein targeting Oxidized Protein Immunoblot Control (Carbonyl-BSA).