Oxiselect Methylglyoxal (Mg) ELISA Kit

Principle of the assay: First, an MG conjugate is coated on the ELISA plate. The unknown MG protein samples or MG-BSA standards are then added to the MG conjugate preabsorbed plate. After a brief incubation, the anti-MG antibody is added, followed by an HRP conjugated secondary antibody. The content of MG protein adducts in unknown samples is determined by comparison with the predetermined MG-BSA standard curve.

Background: The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events. Several AGE structures have been reported, such as Nepsilon-(carboxymethyl) lysine (CML), Nepsilon-(carboxyethyl) lysine (CEL), pentosidine, and Methylglyoxal (MG) derivatives. MG is formed through non-oxidative mechanisms from triose phosphates during anaerobic glycolysis and it can modify amino acids, nucleic acids, and proteins. MG reacts with arginine, lysine and cysteine residues of proteins to form AGEs. MG is involved in various pathological processes. For example, MG derivatives are found elevated in diabetes. The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

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Small volumes of OxiSelect Methylglyoxal (MG) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168996 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OxiSelect Methylglyoxal (MG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OxiSelect Methylglyoxal (MG). The ELISA analytical biochemical technique of the MBS168996 kit is based on OxiSelect Methylglyoxal (MG) antibody-OxiSelect Methylglyoxal (MG) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OxiSelect Methylglyoxal (MG) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OxiSelect Methylglyoxal (MG). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).