Cytoselect 48-Well Cell Adhesion Assay (Collagen IV, Colorimetric) Assay Kit

Principle of the assay: The CytoSelect Cell Adhesion Assay Kit utilizes a Collagen IV-coated 48-well plate (see Adhesion Plate Layout below). First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are washed away, and the adherent cells are fixed/stained. Finally, the stain is extracted and quantified colorimetrically.

Background: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Human Collagen IV-coated wells, 8 BSA-coated wells).

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Small volumes of CytoSelect 48-well Cell Adhesion Assay (Collagen IV, Colorimetric) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169021 is an assay kit targeting CytoSelect 48-well Cell Adhesion Assay (Collagen IV, Colorimetric).