Apoai and Apob Duplex ELISA Kit

Background: Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Plasma apolipoproteins can be grouped into two classes: the nonexchangeable apolipoproteins (ApoB-100 and ApoB-48), and the exchangeable apolipoproteins (ApoAI, ApoAII, ApoAIV, ApoCI, ApoCII, ApoCIII, and ApoE). ApoAI comprises approximately 70% of the protein moiety in HDL (Figure 1). It is a single polypeptide chain consisting of 223 amino acid residues without disulfide bond and with aspartic acid as the the N-terminal residue and glutamic acid as the C-terminal residue. It has an approximate molecular weight 28 kDa. ApoAI activates lecithin-chlesterol (LCAT) acyltransferase, which is responsible for cholesterol esterification in plasma. ApoB-100 is found in lipoproteins originating from the liver (VLDL, IDL, and LDL). Low density lipoprotein (LDL) is the major transport protein for cholesterol in human plasma. LDL is a spherical particle with a diameter of 20-25 nm. Each LDL particle contains cholesteryl esters in its core which are surrounded by a hydrophilic coat composed of phospholipids, cholesterol, and one molecule of a hydrophobic protein known as apolipoprotein B-100 (Figure 1). ApoB-48 is synthesized exclusively by the small intestine. As a result of the RNA editing, ApoB-48 and ApoB-100 share a common N-terminal sequence, but ApoB-48 lacks ApoB-100's C-terminal LDL receptor binding region. ApoB-48 is so called because it constitutes 48% of the sequence for ApoB-100. ApoB-48 is a unique protein to chylomicrons from the small intestine. Human ApoAI and ApoB Duplex ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of both human ApoAI and ApoB (ApoB-100/48) in the same sample of plasma, serum or other biological fluids. The kit has detection sensitivity limit of 0.1 ng/mL (human ApoAI) or 1 ng/mL (human ApoB). Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

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Small volumes of ApoAI and ApoB Duplex elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169123 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ApoAI and ApoB Duplex, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ApoAI and ApoB Duplex. The ELISA analytical biochemical technique of the MBS169123 kit is based on ApoAI and ApoB Duplex antibody-ApoAI and ApoB Duplex antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ApoAI and ApoB Duplex antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ApoAI and ApoB Duplex. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).