Cytoselect 24-Well Cell Haptotaxis Assay (8 Um), COL-Coated, Fluorometric Assay Kit

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect Cell Haptotaxis Assay Kit utilizes polycarbonate membrane inserts (8 mum pore size) to assay the migratory properties of cells, the bottom side of the insert is coated with Collagen I. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 mum pore size is optimal for epithelial and fibroblast cell migration. The kit does not require you to prelabel the cells with Calcein AM. Migratory cells are lysed and detected by the patented CyQuant GR Dye. The CytoSelect Cell Haptotaxis Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards the gradient of extracellular matrix density (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. Finally, the cells are removed from the top of the membrane and the migratory cells are lysed and detected by the patented CyQuant GR Dye.

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3. Lauffenburger DA, Horwitz AF. (1996) Cell 84, 359-369.

Small volumes of CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Fluorometric assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169224 is an assay kit targeting CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Fluorometric.