Ca 15-3 ELISA Kit for Human

Principle of the Assay: The CA15-3 ELISA test is an adapted solid phase sequential sandwich ELISA. Samples and biotinylated monoclonal antibody are added to wells coated with streptavidin. CA15-3 in the patient sample binds to biotinylated capture antibody. The biotinylated antibody simultaneously binds to the streptavidin coated plate. After a wash step, anti-CA15-3-HRP enzyme conjugate is added and forms a sandwich around captured CA15-3. Unbound antibodies are washed off. TMB substrate is added resulting in the development of a blue color. The concentration of CA15-3 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA15-3 concentration.

Intended Uses: The CA15-3 ELISA Kit is intended for the quantitative determination of the cancer antigen CA15-3 concentration in human serum or plasma.

Small volumes of CA 15-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580044 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CA 15-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA 15-3. The ELISA analytical biochemical technique of the MBS580044 kit is based on CA 15-3 antibody-CA 15-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA 15-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA 15-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).