COVID 19 Nucleocapsid (Np) Coronavirus (COVID-19) ELISA Kit

Background/Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel form of coronavirus. SARS-CoV-2 is closely related to the original virus known as SARS-CoV. The protein makeup of SARS-CoV-2 includes membrane glycoprotein (M), envelope protein (E), spike protein (S), and the nucleocapsid protein (N). The N Protein is an RNA binding protein which binds the RNA genome of the virus and packages it into a long helix-containing nucleocapsid structure called a ribonucleoprotein (RNP) complex. The N protein has been shown to be very important for keeping the RNA in a highly ordered conformation in order to facilitate replication and transcription of the viral genome. In addition, the N protein is responsible for regulating interactions with the host such as actin reorganization, apoptosis, and cell cycle regulation in host cells. SARS-CoV-2 Nucleocapsid ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of SARS-CoV-2 N Protein. The kit has a detection sensitivity limit of 1.25 ng/mL N protein. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Small volumes of COVID-19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169598 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COVID 19 Nucleocapsid (NP) Coronavirus (COVID-19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COVID-19. The ELISA analytical biochemical technique of the MBS169598 kit is based on COVID-19 antibody-COVID-19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COVID-19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COVID-19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).