S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) (SAM/SAH) ELISA Kit

Background: S-adenosylmethionine (SAM) is a methyl donor involved in the transfer of a methyl group to DNA, proteins, phospholipids, RNA, and neurotransmitters. Reactions that break down and regenerate SAM have been named the SAM cycle (Figure 1). SAM-dependent methylases use SAM as a substrate to yield s-adenosylhomocysteine (SAH), which is further broken down to homocysteine and adenosine by s-adenosylhomocysteine hydrolase. The homocysteine can be regenerated to methionine and finally SAM by methionine synthases. Donation of the SAM methyl group converts SAM into SAH, the latter being a potent inhibitor of methylation. For this reason, the SAM/SAH ratio has been used as an index of methylation potential in a cell. SAH in plasma has been found to be a more sensitive indicator for vascular disease risk than homocysteine. In addition, distinct classes of riboswitches that recognize SAH but not SAH and regulate gene expression have been discovered. SAM and SAH Combo ELISA Kit is a competitive enzyme immunoassay developed for the detection and quantitation of SAM and SAH in plasma, serum, lysates, or other biological fluid samples. The kit has a detection sensitivity limit of 0.625 nM for free SAM and 0.2 uM for free SAH. Each kit provides sufficient reagents to perform up to 96 total assays for SAM or SAH, including standard curves and unknown samples.

Small volumes of SAM/SAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169604 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) (SAM/SAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SAM/SAH. The ELISA analytical biochemical technique of the MBS169604 kit is based on SAM/SAH antibody-SAM/SAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SAM/SAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SAM/SAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).