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PAK5/6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612591

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits… More details

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Product Overview

Online Datasheet in PDF
Product Name
PAK5/6, Polyclonal Antibody
Full Product Name
Phospho-PAK5/6 (Ser602/Ser560) Antibody
Product Gene Name
Product Synonym Gene Name
PAK6, PAK5
Product Synonym Names
CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21 (CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6; EC 2.7.11.1; KIAA1264; MGC26232; p21 activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21 (CDKN1A) activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; PAK 5; PAK 7; PAK-5; PAK-7; PAK5; PAK7; PAK7_HUMAN; Protein kinase PAK5; Serine/threonine protein kinase PAK 7; Serine/threonine-protein kinase PAK 7
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Concentration
1mg/ml (lot specific)
Specificity
Phospho-PAK5/6 (Ser602/Ser560) Antibody detects endogenous levels of PAK5/6 only when phosphorylated at Ser602/560.
Tissue Specificity: Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Molecular Weight
80,745 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (9518037..9819687, complement). Location: 20p12
OMIM
608038
3D Structure
ModBase 3D Structure for Q9P286
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human PAK5/6 around the phosphorylation site of Ser602/560.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.
Subunit Structure
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.
Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Subcellular Location
Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PAK5/6 antibody
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes for anti-PAK5/6 antibody
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Mouse ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Immunohistochemistry (IHC) of anti-PAK5/6 antibody

At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-PAK5/6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9612591

Other Notes

Small volumes of anti-PAK5/6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAK5/6 purchase

MBS9612591 is an antibody targeting PAK5/6. Also known as Phospho-PAK5/6 (Ser602/Ser560) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAK5/6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase PAK 7
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 7
NCBI Official Symbol
PAK7
NCBI Official Synonym Symbols
PAK5
UniProt Protein Name
Serine/threonine-protein kinase PAK 7
UniProt Synonym Protein Names
p21-activated kinase 5; PAK-5; p21-activated kinase 7
UniProt Gene Name
PAK7
UniProt Synonym Gene Names
KIAA1264; PAK5; PAK-5; PAK-7
UniProt Entry Name
PAK7_HUMAN
NCBI Protein Information
serine/threonine-protein kinase PAK 7; PAK-5; PAK-7; protein kinase PAK5; p21-activated kinase 5; p21-activated kinase 7; p21CDKN1A-activated kinase 7; serine/threonine-protein kinase PAK7

NCBI Summary for PAK5/6

The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for PAK5/6

PAK5: a protein kinase of the STE20 family. Predominantly expressed in brain. Capable of promoting neurite outgrowth, and thus may play a role in neurite development. Associated with microtubule networks and induces microtubule stabilization. Its subcellular localization is tightly regulated during cell cycle progression.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: cell proliferation; cell migration; apoptosis; locomotory behavior; cytoskeleton organization and biogenesis; mitotic cell cycle; learning; cell growth; signal transduction; protein amino acid phosphorylation; memory
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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