Hepcidin (HEPC) ELISA Kit for Rat

Name: Rat HEPC ELISA Kit; Hepcidin; MyBioSource
Brand: MyBioSource
SKU: MBS017183
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS017183

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of HEPC (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Hepcidin, ELISA Kit

Full Product Name

Rat Hepcidin ELISA Kit

Product Gene Name

HEPC elisa kit

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Detection Range

5.0ng/ml-160ng/ml

Sensitivity

1.0ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

9,408 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (35773410..35776046). Location: 19q13.1

OMIM

606464

3D Structure

ModBase 3D Structure for P81172

Other Notes

Small volumes of HEPC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEPC purchase

MBS017183 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepcidin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HEPC. The ELISA analytical biochemical technique of the MBS017183 kit is based on HEPC antibody-HEPC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HEPC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HEPC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Iron is both a requirement and a highly toxic element for life. In human biology, iron is tightly regulated in serum samples by hepcidin. Disease states, like hepatitis virus - HCV low - infections elevate level and result in iron deficiency while low levels in patients causes clinical toxic overload. Therefore, detection by serum hepcidin ELISA for animal disease models like guinea pig hepcidin, mouse hepcidin and rat hepcidin is a powerful research ally. No matter your species our pig hepcidin ELISA, mouse hepcidin ELISA, human hepcidin ELISA or this specific rat hepcidin ELISA is sure to get your data correctly. We supply 1 plate prebound to antibody to test in the assay, along with anti rat secondary labeled antibodies, standard reagent and all needed kit solution stocks. ELISA kit protocol is detailed, like all our ELISA kits, on rt datasheet, included in hepcidin ELISA kit 96 test packages.

Additional Details

NCBI/Uniprot data below describe general gene information for HEPC. It may not necessarily be applicable to this product.

NCBI GI #

10863973

NCBI GeneID

57817

NCBI Accession #

NP_066998.1

UniProt Primary Accession #

P81172

UniProt Secondary Accession #

Q1HE14; Q9BY68

UniProt Related Accession #

P81172

GenBank Nucleotide #

NM_021175.2

NCBI Official Full Name

hepcidin preproprotein

NCBI Official Synonym Full Names

hepcidin antimicrobial peptide

NCBI Official Symbol

HAMP

NCBI Official Synonym Symbols

HEPC; PLTR; HFE2B; LEAP1

UniProt Protein Name

Hepcidin

UniProt Synonym Protein Names

Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor

Protein Family

Heparin-sulfate lyase

UniProt Gene Name

HAMP

UniProt Synonym Gene Names

HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20

UniProt Entry Name

HEPC_HUMAN

NCBI Protein Information

hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1

NCBI Summary for HEPC

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

UniProt Comments for HEPC

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: killing of cells of another organism; cellular iron ion homeostasis; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b

Research Articles on HEPC

1. In vitro studies showed a minimal upregulation of hepcidin mRNA in HepG2 cells treated with hereditary hemochromatosis patients' serum.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HEPC elisa kit

Reference Product	PubMed Publications
TFR2 elisa kit	>56 publications with HEPC and TFR2
HFE2 elisa kit	>33 publications with HEPC and HFE2
SLC40A1 elisa kit	>31 publications with HEPC and SLC40A1
TMPRSS6 elisa kit	>9 publications with HEPC and TMPRSS6
STAT3 elisa kit	>6 publications with HEPC and STAT3
SLC11A2 elisa kit	>4 publications with HEPC and SLC11A2
IL6 elisa kit	>2 publications with HEPC and IL6
GDF15 elisa kit	>2 publications with HEPC and GDF15

Pathways associated with HEPC elisa kit

Products by Pathway	Pathway Diagram
Iron Metabolism In Placenta Pathway antibodies	Iron Metabolism In Placenta Pathway Diagram

Diseases associated with HEPC elisa kit

Disease Name	Pubmed Publications
Iron Overload Antibodies	>105 publications with HEPC and Iron Overload
Hemochromatosis Antibodies	>85 publications with HEPC and Hemochromatosis
Anemia Antibodies	>40 publications with HEPC and Anemia
Liver Diseases Antibodies	>27 publications with HEPC and Liver Diseases
Inflammation Antibodies	>26 publications with HEPC and Inflammation
beta-Thalassemia Antibodies	>10 publications with HEPC and beta-Thalassemia
Neoplasms Antibodies	>10 publications with HEPC and Neoplasms
Drug Toxicity Antibodies	>8 publications with HEPC and Drug Toxicity
Liver Neoplasms Antibodies	>6 publications with HEPC and Liver Neoplasms
Disease Models, Animal Antibodies	>6 publications with HEPC and Disease Models, Animal