Calf Intestinal Alkaline Phosphatase (ALPI) Active Protein

Name: ALPI Active Protein; Alkaline Phosphatase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS173171
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS173171

This ALPI (Calf Intestinal Alkaline Phosphatase) active protein is a hydrolase enzyme supplied in bulk-quantity formats, supporting molecular biology dephosphorylation applications and enzyme research. MyBioSource is the leading producer of Calf Intestine… More details

$460 / 10K Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alkaline Phosphatase (ALPI), Active Protein

Full Product Name

Alkaline Phosphatase (CIP/CIAP), Calf Intestinal

Product Gene Name

ALPI active protein

Product Synonym Gene Name

BOS_2150; IAP

Product Synonym Names

Alk Phos; CIP; CIAP; ALP; Orthophosphoric-Monoester Phosphohydrolase; Alkaline Phosphatase (CIP/CIAP), Calf Intestinal - Lyophilized

Form/Format

Lyophilized powder with magnesium chloride.

Purity/Purification

Purified

Preparation and Storage

Aliquot and freeze at -20 degree C after reconstitution. Avoid freeze thaw cycles.
Note: Protect from moisture
Advice: Reconstitute into provided container and centrifuge to ensure full recovery.
Handling: Handle as a potentially hazardous substance.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source

Calf Intestines

EC Number

3.1.3.1

Unit Definition

One unit will convert on micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37 degree C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.25 and 37 degree C.

Specific Activity

1390 units/mg protein

Appearance

Tan powder

Solubility

Clear, colorless in 1 mg/mL saline.

Protein

0.48 mg protein/mg (Coomassie)

Contaminants

Ammonia: <0.1 micromole/mg

CAS Number

9001-78-9

Activity

660 Units/mg (Dimension Clinical Chemistry System)

Reconstitution

Tris buffered saline with 1 mM magnesium chloride, 0.2mM zinc chloride, and 1% BSA, pH8.0

Product Categories/Family for ALPI active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Alkaline Phosphatase (cip/ciap)

Other Notes

Small volumes of ALPI active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALPI purchase

Alkaline phosphatase is an enzyme hydrolase, with human protein expression high in many cell subtypes and tissue types, exemplified by liver, bone and pluripotent stem cell differentiation. Full genes of human mouse rat ALPI DNA sequence are found in NBCI Gene database. Uniprotkb for href and Pubmed protein databases are services to target further information about intestinal alkaline phosphatase proteins and publications box summaries. Uses for CIP/CIAP include dephosphoryation of vectors in ligation reactions, assessing bone loss in disease or from complications of drugs compounds and evaluating quality of heat pasteurization. EC# 3 1 31 is available as a stable lyophilized protein in multiple quantities products at competitive pricing, long shelf life and high specific activity of _= 280 in U/mg protein when p-nitrophenylphosphate and AMP (2-amino-2-methyl-1-propanol) are used as substrate. Other potential applications, such as antibody production, are included in one section of the technical manual provided

Additional Details

NCBI/Uniprot data below describe general gene information for ALPI. It may not necessarily be applicable to this product.

NCBI GI #

110350019

NCBI GeneID

727689

NCBI Accession #

NP_001036028.1

UniProt Related Accession #

Q29486

GenBank Nucleotide #

NM_001042563.1

NCBI Official Full Name

alkaline phosphatase, tissue-nonspecific isozyme

NCBI Official Symbol

ALPL

UniProt Protein Name

Alkaline phosphatase, tissue-nonspecific isozyme

UniProt Synonym Protein Names

Alkaline phosphatase liver/bone/kidney isozyme

Protein Family

Intestinal-type alkaline phosphatase

UniProt Gene Name

ALPL

UniProt Synonym Gene Names

AP-TNAP; TNSALP

UniProt Entry Name

PPBT_FELCA

NCBI Protein Information

alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; AP-TNAP; alkaline phosphatase liver/bone/kidney isozyme

UniProt Comments for ALPI

This isozyme may play a role in skeletal mineralization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ALPI active protein

Reference Product	PubMed Publications
PTH active protein	>5 publications with ALPI and PTH
TNF active protein	>1 publications with ALPI and TNF
ACPP active protein	>1 publications with ALPI and ACPP

Pathways associated with ALPI active protein

Products by Pathway	Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram

Diseases associated with ALPI active protein

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>12 publications with ALPI and Kidney Diseases
Seizures Antibodies	>11 publications with ALPI and Seizures
Cardiovascular Diseases Antibodies	>11 publications with ALPI and Cardiovascular Diseases
Pain Antibodies	>9 publications with ALPI and Pain
Inflammation Antibodies	>8 publications with ALPI and Inflammation
Calcinosis Antibodies	>5 publications with ALPI and Calcinosis
Liver Diseases Antibodies	>5 publications with ALPI and Liver Diseases
Necrosis Antibodies	>4 publications with ALPI and Necrosis
Hypertension Antibodies	>4 publications with ALPI and Hypertension
Hypertrophy Antibodies	>4 publications with ALPI and Hypertrophy