Perforin 1 (PRF1) ELISA Kit for Human

Name: Human PRF1 ELISA Kit; Perforin 1; MyBioSource
Brand: MyBioSource
SKU: MBS017462
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS017462

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of PRF1 (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Perforin 1 (PRF1), ELISA Kit

Full Product Name

Human Perforin 1 ELISA Kit

Product Gene Name

PRF1 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Detection Range

31.2pg/ml-1000pg/ml

Sensitivity

5.0pg/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

61,377 Da

Chromosome Location

Chromosome: 10; NC_000010.10 (72357104..72362531, complement). Location: 10q22

OMIM

170280

3D Structure

ModBase 3D Structure for P14222

Other Notes

Small volumes of PRF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRF1 purchase

MBS017462 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Perforin 1 (PRF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRF1. The ELISA analytical biochemical technique of the MBS017462 kit is based on PRF1 antibody-PRF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRF1. It may not necessarily be applicable to this product.

NCBI GI #

133908621

NCBI GeneID

5551

NCBI Accession #

NP_001076585.1

UniProt Primary Accession #

P14222

UniProt Secondary Accession #

Q59F57; Q86WX7; B2R6X4

UniProt Related Accession #

P14222

GenBank Nucleotide #

NM_001083116.1

NCBI Official Full Name

perforin-1

NCBI Official Synonym Full Names

perforin 1 (pore forming protein)

NCBI Official Symbol

PRF1

NCBI Official Synonym Symbols

P1; PFP; FLH2; PFN1; HPLH2

UniProt Protein Name

Perforin-1

UniProt Synonym Protein Names

Cytolysin; Lymphocyte pore-forming protein

Protein Family

Peptide chain release factor

UniProt Gene Name

PRF1

UniProt Synonym Gene Names

PFP; P1; PFP

UniProt Entry Name

PERF_HUMAN

NCBI Protein Information

perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein

NCBI Summary for PRF1

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

UniProt Comments for PRF1

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; transmembrane transport; defense response to virus; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2

Research Articles on PRF1

1. The mRNA and protein expression of perforin significantly increases in CD4(+) and CD8(+) T cells treated with 5-azaC, which is associated with DNA hypomethylation of perforin promoter in T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRF1 elisa kit

Reference Product	PubMed Publications
GZMB elisa kit	>526 publications with PRF1 and GZMB
IL2RA elisa kit	>42 publications with PRF1 and IL2RA
CD86 elisa kit	>38 publications with PRF1 and CD86
EOMES elisa kit	>19 publications with PRF1 and EOMES
IL2 elisa kit	>13 publications with PRF1 and IL2
IL2RB elisa kit	>12 publications with PRF1 and IL2RB
CD3D elisa kit	>3 publications with PRF1 and CD3D
CD3E elisa kit	>1 publications with PRF1 and CD3E
CD247 elisa kit	>1 publications with PRF1 and CD247

Pathways associated with PRF1 elisa kit

Products by Pathway	Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram
IL12 Signaling Mediated By STAT4 Pathway antibodies	IL12 Signaling Mediated By STAT4 Pathway Diagram

Diseases associated with PRF1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>962 publications with PRF1 and Neoplasms
Immune System Diseases Antibodies	>893 publications with PRF1 and Immune System Diseases
Necrosis Antibodies	>655 publications with PRF1 and Necrosis
Neoplasms, Experimental Antibodies	>273 publications with PRF1 and Neoplasms, Experimental
Inflammation Antibodies	>267 publications with PRF1 and Inflammation
Carcinoma Antibodies	>193 publications with PRF1 and Carcinoma
Liver Diseases Antibodies	>172 publications with PRF1 and Liver Diseases
Lymphoma, Non-Hodgkin Antibodies	>152 publications with PRF1 and Lymphoma, Non-Hodgkin
Cardiovascular Diseases Antibodies	>139 publications with PRF1 and Cardiovascular Diseases
Drug Toxicity Antibodies	>89 publications with PRF1 and Drug Toxicity

Organs/Tissues associated with PRF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3687 publications with PRF1 and Blood
Liver Antibodies	>305 publications with PRF1 and Liver
Spleen Antibodies	>297 publications with PRF1 and Spleen
Bone Marrow Antibodies	>249 publications with PRF1 and Bone Marrow
Lung Antibodies	>218 publications with PRF1 and Lung
Kidney Antibodies	>183 publications with PRF1 and Kidney
Lymph Node Antibodies	>175 publications with PRF1 and Lymph Node
Brain Antibodies	>140 publications with PRF1 and Brain
Muscle Antibodies	>123 publications with PRF1 and Muscle
Intestine Antibodies	>97 publications with PRF1 and Intestine