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JNK3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750010

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated… More details

$210 / 0.03 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
JNK3, Monoclonal Antibody
Full Product Name
Anti-JNK3 Rabbit Monoclonal Antibody
Product Gene Name
anti-JNK3 antibody
Product Synonym Gene Name
MAPK10
Product Synonym Names
Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; 2.7.11.24; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAPK10; JNK3; JNK3A; PRKM10; SAPK1B
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
52585 MW
OMIM
U07620 mRNA
3D Structure
ModBase 3D Structure for P53779
Immunogen
A synthesized peptide derived from human JNK3
Subcellular Localization
Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion. Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity).
Tissue Specificity
Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-JNK3 antibody

Signal Transduction; Protein Phosphorylation; SER/THR Kinases; MAPK Pathway; Cancer; Serine/Threonine Kinases; Oncoproteins/Suppressors; Oncoproteins; signal transducers; Immunology; Innate Immunity; TLR Signaling
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-JNK3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-JNK3 antibody
WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:500

Western Blot (WB) of anti-JNK3 antibody

Western blot analysis of JNK3 expression in HeLa lysate (MBS1750010).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK10 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK10
Rabbit Anti-JNK3 Monoclonal Antibody – Cat# MBS1750010

Other Notes

Small volumes of anti-JNK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JNK3 purchase

MBS1750010 is an antibody targeting JNK3. Also known as Anti-JNK3 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JNK3. It may not necessarily be applicable to this product.

NCBI GI #
969536252
NCBI GeneID
5602
NCBI Accession #
NP_001304996.1
UniProt Primary Accession #
P53779
UniProt Secondary Accession #
Q15707; Q49AP1; A6NFS3; A6NG28; B3KQ94
UniProt Related Accession #
P53779
GenBank Nucleotide #
NM_001318067.1
NCBI Official Full Name
mitogen-activated protein kinase 10 isoform 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase 10
NCBI Official Symbol
MAPK10
NCBI Official Synonym Symbols
JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
UniProt Protein Name
Mitogen-activated protein kinase 10
UniProt Synonym Protein Names
MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3
UniProt Gene Name
MAPK10
UniProt Synonym Gene Names
JNK3; JNK3A; PRKM10; SAPK1B; MAP kinase 10; MAPK 10; SAPK1b
NCBI Protein Information
mitogen-activated protein kinase 10

NCBI Summary for JNK3

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]
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UniProt Comments for JNK3

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692).
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Research Articles on JNK3

1. Mapk10 expression was regulated by miR27a-3p in nasopharyngeal carcinoma.Mapk10 gene was down-regulated in the nasopharyngeal carcinoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JNK3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >6 publications with JNK3 and JUN
 DUSP16 antibody  >1 publications with JNK3 and DUSP16
 FOS antibody  >1 publications with JNK3 and FOS

Pathways associated with anti-JNK3 antibody

 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Angiopoietin Like Protein 8 Regulatory Pathway antibodies  Angiopoietin Like Protein 8 Regulatory Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram

Diseases associated with anti-JNK3 antibody

 Disease Name  Pubmed Publications
 Chromosome Aberrations Antibodies  >3 publications with JNK3 and Chromosome Aberrations
 Lung Diseases Antibodies  >2 publications with JNK3 and Lung Diseases
 Heart Diseases Antibodies  >2 publications with JNK3 and Heart Diseases
 Urinary Bladder Neoplasms Antibodies  >1 publications with JNK3 and Urinary Bladder Neoplasms
 Hypertension Antibodies  >1 publications with JNK3 and Hypertension
 Lung Neoplasms Antibodies  >1 publications with JNK3 and Lung Neoplasms
 Edema Antibodies  >1 publications with JNK3 and Edema
 Kidney Diseases Antibodies  >1 publications with JNK3 and Kidney Diseases
 Breast Neoplasms Antibodies  >1 publications with JNK3 and Breast Neoplasms
 Pulmonary Edema Antibodies  >1 publications with JNK3 and Pulmonary Edema

Organs/Tissues associated with anti-JNK3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >4 publications with JNK3 and Muscle
 Heart Antibodies  >4 publications with JNK3 and Heart
 Lung Antibodies  >2 publications with JNK3 and Lung
 Nerve Antibodies  >2 publications with JNK3 and Nerve
 Brain Antibodies  >2 publications with JNK3 and Brain
 Bone Antibodies  >1 publications with JNK3 and Bone
 Bladder Antibodies  >1 publications with JNK3 and Bladder
 Intestine Antibodies  >1 publications with JNK3 and Intestine
 Embryonic Tissue Antibodies  >1 publications with JNK3 and Embryonic Tissue
 Pancreas Antibodies  >1 publications with JNK3 and Pancreas
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