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ADFP Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750012

MBS1750012 is an antibody targeting ADFP. Also known as Anti-ADFP Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. May be involved in development and maintenance of adipose tissue. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADFP, Monoclonal Antibody
Full Product Name
Anti-ADFP Rabbit Monoclonal Antibody
Product Gene Name
anti-ADFP antibody
Product Synonym Gene Name
PLIN2
Product Synonym Names
Perilipin-2; Adipophilin; Adipose differentiation-related protein; ADRP; PLIN2; ADFP
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
48075 MW
OMIM
X97324 mRNA
3D Structure
ModBase 3D Structure for Q99541
Immunogen
A synthesized peptide derived from human ADFP
Subcellular Localization
Membrane; Peripheral membrane protein.
Tissue Specificity
Milk lipid globules.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ADFP antibody

Cardiovascular; Hypoxia; Hypoxia-Regulated; Cardiovascular; Lipids/Lipoproteins; Adipose Related; Lipid Droplet Protein; Signal Transduction; Metabolism; Lipid Metabolism; Atherosclerosis; Lipid Transport; Cancer; Cancer Metabolism; Metabolic Signaling Pa
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ADFP antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ADFP antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-ADFP antibody

Western blot analysis of ADFP expression in HepG2 cell lysate (MBS1750012).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLIN2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLIN2
Rabbit Anti-ADFP Monoclonal Antibody – Cat# MBS1750012

Other Notes

Small volumes of anti-ADFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADFP. It may not necessarily be applicable to this product.

NCBI GI #
34577059
NCBI GeneID
123
NCBI Accession #
NP_001113.2
UniProt Primary Accession #
Q99541
UniProt Secondary Accession #
Q9BSC3
UniProt Related Accession #
Q99541
GenBank Nucleotide #
NM_001122.3
NCBI Official Full Name
perilipin-2
NCBI Official Synonym Full Names
perilipin 2
NCBI Official Symbol
PLIN2
NCBI Official Synonym Symbols
ADFP; ADRP
UniProt Protein Name
Perilipin-2
UniProt Synonym Protein Names
Adipophilin; Adipose differentiation-related protein; ADRP
UniProt Gene Name
PLIN2
UniProt Synonym Gene Names
ADFP; ADRP
NCBI Protein Information
perilipin-2

NCBI Summary for ADFP

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
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UniProt Comments for ADFP

May be involved in development and maintenance of adipose tissue.

Research Articles on ADFP

1. PLIN2 gene Ser251Pro missense mutation is associated with reduced insulin secretion and increased insulin sensitivity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADFP antibody

 Reference Product  PubMed Publications
 PPARA antibody  >2 publications with ADFP and PPARA
 CARM1 antibody  >1 publications with ADFP and CARM1
 RXRA antibody  >1 publications with ADFP and RXRA

Pathways associated with anti-ADFP antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram

Diseases associated with anti-ADFP antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >12 publications with ADFP and Fatty Liver
 Liver Diseases Antibodies  >7 publications with ADFP and Liver Diseases
 Inflammation Antibodies  >5 publications with ADFP and Inflammation
 Kidney Diseases Antibodies  >4 publications with ADFP and Kidney Diseases
 Adenocarcinoma Antibodies  >3 publications with ADFP and Adenocarcinoma
 Atrophy Antibodies  >2 publications with ADFP and Atrophy
 Breast Neoplasms Antibodies  >2 publications with ADFP and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >2 publications with ADFP and Cardiovascular Diseases
 Fibrosis Antibodies  >1 publications with ADFP and Fibrosis
 Weight Loss Antibodies  >1 publications with ADFP and Weight Loss

Organs/Tissues associated with anti-ADFP antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >21 publications with ADFP and Muscle
 Liver Antibodies  >14 publications with ADFP and Liver
 Blood Antibodies  >12 publications with ADFP and Blood
 Adipose Tissue Antibodies  >12 publications with ADFP and Adipose Tissue
 Mammary Gland Antibodies  >5 publications with ADFP and Mammary Gland
 Bone Antibodies  >5 publications with ADFP and Bone
 Connective Tissue Antibodies  >4 publications with ADFP and Connective Tissue
 Kidney Antibodies  >4 publications with ADFP and Kidney
 Heart Antibodies  >2 publications with ADFP and Heart
 Skin Antibodies  >2 publications with ADFP and Skin
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