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Rab4A/RAB4 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750026

MBS1750026 is an antibody targeting Rab4A/Rab4. Also known as Anti-Rab4A/Rab4 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Protein transport. Probably involved in vesicular traffic (By similarity). More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rab4A/Rab4, Monoclonal Antibody
Full Product Name
Anti-Rab4A/Rab4 Rabbit Monoclonal Antibody
Product Gene Name
anti-Rab4A/Rab4 antibody
Product Synonym Gene Name
RAB4A
Product Synonym Names
Ras-related protein Rab-4A; RAB4A; RAB4
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
24390 MW
OMIM
M28211 mRNA
3D Structure
ModBase 3D Structure for P20338
Immunogen
A synthesized peptide derived from human Rab4A
Subcellular Localization
Membrane; Peripheral membrane protein. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Rab4A/Rab4 antibody

Tags & Cell Markers; Subcellular Markers; organelles; endosome; Signal Transduction; Signaling Pathway; G Protein Signaling; Small G Proteins; Protein Trafficking; Vesicle Transport; regulation; Neuroscience; Cell Type Marker; neuron marker; synapse marke
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Rab4A/Rab4 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-Rab4A/Rab4 antibody
WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-Rab4A/Rab4 antibody

Western blot analysis of Rab4A expression in (1) SH-SY5Y cell lysate; (2) MCF-7 cell lysate (MBS1750026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB4A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB4A
Rabbit Anti-Rab4A/RAB4 Monoclonal Antibody – Cat# MBS1750026

Other Notes

Small volumes of anti-Rab4A/Rab4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Rab4A/Rab4. It may not necessarily be applicable to this product.

NCBI GI #
433660831
NCBI GeneID
5867
NCBI Accession #
NP_001258927.1
UniProt Primary Accession #
P20338
UniProt Secondary Accession #
Q5T7P7; Q9BQ44
UniProt Related Accession #
P20338
GenBank Nucleotide #
NM_001271998.1
NCBI Official Full Name
ras-related protein Rab-4A isoform 2
NCBI Official Synonym Full Names
RAB4A, member RAS oncogene family
NCBI Official Symbol
RAB4A
NCBI Official Synonym Symbols
RAB4; HRES1; HRES-1; HRES-1/RAB4
UniProt Protein Name
Ras-related protein Rab-4A
UniProt Gene Name
RAB4A
UniProt Synonym Gene Names
RAB4
NCBI Protein Information
ras-related protein Rab-4A

NCBI Summary for Rab4A/Rab4

This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
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UniProt Comments for Rab4A/Rab4

Protein transport. Probably involved in vesicular traffic ().

Research Articles on Rab4A/Rab4

1. These findings bring attention to the effects of C-terminal carboxylmethylation on RAB GTPases and provide a rationale for targeting ICMT in the treatment of metastatic cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rab4A/Rab4 antibody

 Reference Product  PubMed Publications
 PRKCA antibody  >1 publications with Rab4A/Rab4 and PRKCA
 RUFY1 antibody  >1 publications with Rab4A/Rab4 and RUFY1
 RABEP1 antibody  >1 publications with Rab4A/Rab4 and RABEP1

Pathways associated with anti-Rab4A/Rab4 antibody

 Products by Pathway  Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Signaling Events Mediated By TCPTP Pathway antibodies  Signaling Events Mediated By TCPTP Pathway Diagram
 Translocation Of GLUT4 To The Plasma Membrane Pathway antibodies  Translocation Of GLUT4 To The Plasma Membrane Pathway Diagram

Diseases associated with anti-Rab4A/Rab4 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3 publications with Rab4A/Rab4 and Cardiovascular Diseases
 Diabetes Mellitus Antibodies  >3 publications with Rab4A/Rab4 and Diabetes Mellitus
 Heart Diseases Antibodies  >3 publications with Rab4A/Rab4 and Heart Diseases
 Hypertrophy Antibodies  >3 publications with Rab4A/Rab4 and Hypertrophy
 Breast Neoplasms Antibodies  >2 publications with Rab4A/Rab4 and Breast Neoplasms
 Hypertension Antibodies  >1 publications with Rab4A/Rab4 and Hypertension
 Heart Failure Antibodies  >1 publications with Rab4A/Rab4 and Heart Failure
 Liver Diseases Antibodies  >1 publications with Rab4A/Rab4 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with Rab4A/Rab4 and Kidney Diseases
 Immune System Diseases Antibodies  >1 publications with Rab4A/Rab4 and Immune System Diseases

Organs/Tissues associated with anti-Rab4A/Rab4 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >9 publications with Rab4A/Rab4 and Heart
 Muscle Antibodies  >8 publications with Rab4A/Rab4 and Muscle
 Blood Antibodies  >4 publications with Rab4A/Rab4 and Blood
 Liver Antibodies  >3 publications with Rab4A/Rab4 and Liver
 Vascular Antibodies  >3 publications with Rab4A/Rab4 and Vascular
 Brain Antibodies  >3 publications with Rab4A/Rab4 and Brain
 Kidney Antibodies  >2 publications with Rab4A/Rab4 and Kidney
 Nerve Antibodies  >1 publications with Rab4A/Rab4 and Nerve
 Testis Antibodies  >1 publications with Rab4A/Rab4 and Testis
 Prostate Antibodies  >1 publications with Rab4A/Rab4 and Prostate
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