• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Alpha Sarcoglycan (SGCA) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750028

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
alpha Sarcoglycan (SGCA), Monoclonal Antibody
Full Product Name
Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody
Product Gene Name
anti-SGCA antibody
Product Synonym Names
Alpha-sarcoglycan; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2; SGCA; ADL; DAG2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
42875 MW
OMIM
U08895 mRNA
3D Structure
ModBase 3D Structure for Q16586
Immunogen
A synthesized peptide derived from human alpha Sarcoglycan
Subcellular Localization
Cell membrane, sarcolemma; Single-pass type I membrane protein. Cytoplasm, cytoskeleton.
Tissue Specificity
Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SGCA antibody

Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; glucosaminoglycans
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SGCA antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SGCA antibody
WB: 1:500-1:2000
IHC: 1:100-1:500
IP: 1:40

Western Blot (WB) of anti-SGCA antibody

Western blot analysis of alpha Sarcoglycan expression in Human skeletal muscle lysate (MBS1750028).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SGCA
Rabbit Anti-SGCA Monoclonal Antibody – Cat# MBS1750028

Other Notes

Small volumes of anti-SGCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SGCA purchase

MBS1750028 is an antibody targeting SGCA. Also known as Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SGCA. It may not necessarily be applicable to this product.

NCBI GI #
4506911
NCBI GeneID
6442
NCBI Accession #
NP_000014.1
UniProt Primary Accession #
Q16586
UniProt Secondary Accession #
Q13710; Q13712; A6NEB8; A8K3K7
UniProt Related Accession #
Q16586
GenBank Nucleotide #
NM_000023.3
NCBI Official Full Name
alpha-sarcoglycan isoform 1
NCBI Official Synonym Full Names
sarcoglycan alpha
NCBI Official Symbol
SGCA
NCBI Official Synonym Symbols
ADL; DAG2; 50DAG; DMDA2; LGMD2D; SCARMD1; adhalin
UniProt Protein Name
Alpha-sarcoglycan
UniProt Synonym Protein Names
50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Protein Family
Alpha-sarcoglycan
UniProt Gene Name
SGCA
UniProt Synonym Gene Names
ADL; DAG2; Alpha-SG; 50DAG
NCBI Protein Information
alpha-sarcoglycan

NCBI Summary for SGCA

This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Show more

Research Articles on SGCA

1. Pathogenic mutations were found in SGCA from Egyptian families with limb-girdle muscular dystrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SGCA antibody

 Reference Product  PubMed Publications
 SGCB antibody  >10 publications with SGCA and SGCB
 SGCD antibody  >9 publications with SGCA and SGCD

Pathways associated with anti-SGCA antibody

 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Viral Myocarditis Pathway antibodies  Viral Myocarditis Pathway Diagram

Diseases associated with anti-SGCA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >38 publications with SGCA and Nervous System Diseases
 Neoplasms Antibodies  >18 publications with SGCA and Neoplasms
 Astrocytoma Antibodies  >16 publications with SGCA and Astrocytoma
 Brain Diseases Antibodies  >15 publications with SGCA and Brain Diseases
 Central Nervous System Neoplasms Antibodies  >14 publications with SGCA and Central Nervous System Neoplasms
 Neoplasms, Neuroepithelial Antibodies  >14 publications with SGCA and Neoplasms, Neuroepithelial
 Necrosis Antibodies  >4 publications with SGCA and Necrosis
 Ependymoma Antibodies  >3 publications with SGCA and Ependymoma
 Heart Diseases Antibodies  >3 publications with SGCA and Heart Diseases
 Fibrosis Antibodies  >2 publications with SGCA and Fibrosis

Organs/Tissues associated with anti-SGCA antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with SGCA and Muscle
 Brain Antibodies  >17 publications with SGCA and Brain
 Heart Antibodies  >3 publications with SGCA and Heart
 Embryonic Tissue Antibodies  >3 publications with SGCA and Embryonic Tissue
 Lung Antibodies  >2 publications with SGCA and Lung
 Bone Antibodies  >1 publications with SGCA and Bone
 Connective Tissue Antibodies  >1 publications with SGCA and Connective Tissue
 Eye Antibodies  >1 publications with SGCA and Eye
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000