Rabbit Anti-TK1 Monoclonal Antibody – Cat# MBS1750029

Western blot analysis of Thymidine Kinase 1 expression in Molt-4 cell lysate (MBS1750029).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TK1

Thymidine Kinase 1 (TK1) Antibody – Rabbit Monoclonal

Name: TK1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750029
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750029

MBS1750029 is an antibody targeting TK1. Also known as Anti-Thymidine Kinase 1 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Thymidine Kinase 1 (TK1), Monoclonal Antibody

Full Product Name

Anti-Thymidine Kinase 1 Rabbit Monoclonal Antibody

Product Gene Name

anti-TK1 antibody

Product Synonym Names

Thymidine kinase; cytosolic; 2.7.1.21; TK1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

25469 MW

OMIM

K02581 mRNA

3D Structure

ModBase 3D Structure for P04183

Immunogen

A synthesized peptide derived from human Thymidine Kinase 1

Subcellular Localization

Cytoplasm.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-TK1 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; dna synthesis

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TK1 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-TK1 antibody

WB: 1:5000-1:20000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:20
FC/FACS: 1:50

Other Notes

Small volumes of anti-TK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TK1. It may not necessarily be applicable to this product.

NCBI GI #

164698438

NCBI GeneID

7083

NCBI Accession #

NP_003249.3

UniProt Primary Accession #

P04183

UniProt Secondary Accession #

Q969V0; Q9UMG9; B2RC58

UniProt Related Accession #

P04183

GenBank Nucleotide #

NM_003258.4

NCBI Official Full Name

thymidine kinase, cytosolic isoform 1

NCBI Official Synonym Full Names

thymidine kinase 1

NCBI Official Symbol

TK1

NCBI Official Synonym Symbols

TK2

UniProt Protein Name

Thymidine kinase, cytosolic

Protein Family

Thymidine kinase

UniProt Gene Name

TK1

NCBI Protein Information

thymidine kinase, cytosolic

NCBI Summary for TK1

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

UniProt Comments for TK1

MiscellaneousTwo forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Research Articles on TK1

1. The results have implications for the mechanism by which Fhit regulates TK1 mRNA, and more broadly, for its modulation of multiple functions as tumor suppressor/genome caretaker.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TK1 antibody

Reference Product	PubMed Publications
TP53 antibody	>8 publications with TK1 and TP53
CDKN1A antibody	>2 publications with TK1 and CDKN1A
GAPDH antibody	>1 publications with TK1 and GAPDH
CDK1 antibody	>1 publications with TK1 and CDK1

Pathways associated with anti-TK1 antibody

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Salvage Reactions Pathway antibodies	Pyrimidine Salvage Reactions Pathway Diagram

Diseases associated with anti-TK1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>142 publications with TK1 and Neoplasms
Carcinoma Antibodies	>44 publications with TK1 and Carcinoma
Neoplasms, Experimental Antibodies	>26 publications with TK1 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>25 publications with TK1 and Breast Neoplasms
Lung Neoplasms Antibodies	>20 publications with TK1 and Lung Neoplasms
Drug Toxicity Antibodies	>19 publications with TK1 and Drug Toxicity
Lung Diseases Antibodies	>17 publications with TK1 and Lung Diseases
Adenocarcinoma Antibodies	>17 publications with TK1 and Adenocarcinoma
Disease Models, Animal Antibodies	>12 publications with TK1 and Disease Models, Animal
Brain Diseases Antibodies	>10 publications with TK1 and Brain Diseases

Organs/Tissues associated with anti-TK1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>96 publications with TK1 and Blood
Lung Antibodies	>26 publications with TK1 and Lung
Brain Antibodies	>19 publications with TK1 and Brain
Liver Antibodies	>17 publications with TK1 and Liver
Lymph Antibodies	>15 publications with TK1 and Lymph
Lymph Node Antibodies	>15 publications with TK1 and Lymph Node
Bone Antibodies	>11 publications with TK1 and Bone
Muscle Antibodies	>8 publications with TK1 and Muscle
Bone Marrow Antibodies	>7 publications with TK1 and Bone Marrow
Kidney Antibodies	>7 publications with TK1 and Kidney