PUMA Antibody – Rabbit Monoclonal

Western blot analysis of PUMA using anti-PUMA antibody (MBS1750030).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA antigen affinity purified monoclonal antibody (Catalog # MBS1750030) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PUMA at approximately 21 kDa. The expected band size for PUMA is at 21 kDa.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PUMA Antibody.

Immunofluorescent analysis of Hela cells, using PUMA Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution

Immunofluorescent analysis using the Antibody at 1:150 dilution

Immunofluorescent analysis using the Antibody at 1:500 dilution