Rabbit Anti-CASP14 Monoclonal Antibody – Cat# MBS1750040

Western blot analysis of Caspase-14 expression in Human skin lysate (MBS1750040).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP14 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP14

Caspase-14 (CASP14) Antibody – Rabbit Monoclonal

Name: CASP14 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750040
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750040

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Caspase-14 (CASP14), Monoclonal Antibody

Full Product Name

Anti-Caspase-14 Rabbit Monoclonal Antibody

Product Gene Name

anti-CASP14 antibody

Product Synonym Names

Caspase-14; CASP-14; 3.4.22.-; Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form; CASP14

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

27680 MW

OMIM

AF097874 mRNA

3D Structure

ModBase 3D Structure for P31944

Immunogen

A synthesized peptide derived from human Caspase-14

Subcellular Localization

Cytoplasm. Nucleus.

Tissue Specificity

Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed: 11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed: 22825846).

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CASP14 antibody

Cell Biology; Apoptosis; Intracellular; Caspases Etc; Caspases

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CASP14 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-CASP14 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Other Notes

Small volumes of anti-CASP14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASP14 purchase

MBS1750040 is an antibody targeting CASP14. Also known as Anti-Caspase-14 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CASP14. It may not necessarily be applicable to this product.

NCBI GI #

6912286

NCBI GeneID

23581

NCBI Accession #

NP_036246.1

UniProt Primary Accession #

P31944

UniProt Secondary Accession #

O95823; Q3SYC9

UniProt Related Accession #

P31944

GenBank Nucleotide #

NM_012114.2

NCBI Official Full Name

caspase-14

NCBI Official Synonym Full Names

caspase 14

NCBI Official Symbol

CASP14

NCBI Official Synonym Symbols

ARCI12

UniProt Protein Name

Caspase-14

Protein Family

Caspase

UniProt Gene Name

CASP14

UniProt Synonym Gene Names

CASP-14

NCBI Protein Information

caspase-14

NCBI Summary for CASP14

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

UniProt Comments for CASP14

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).

Research Articles on CASP14

1. High CASP14 expression is a marker of breast cancer aggressiveness in association with proliferation, TNBC phenotype, and cancer stemness.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CASP14 antibody

Reference Product	PubMed Publications
CASP8 antibody	>4 publications with CASP14 and CASP8
CASP10 antibody	>2 publications with CASP14 and CASP10
CASP1 antibody	>2 publications with CASP14 and CASP1

Diseases associated with anti-CASP14 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with CASP14 and Neoplasms
Brain Diseases Antibodies	>4 publications with CASP14 and Brain Diseases
Skin Neoplasms Antibodies	>3 publications with CASP14 and Skin Neoplasms
Cardiovascular Diseases Antibodies	>3 publications with CASP14 and Cardiovascular Diseases
Necrosis Antibodies	>2 publications with CASP14 and Necrosis
Inflammation Antibodies	>2 publications with CASP14 and Inflammation
Fetal Death Antibodies	>1 publications with CASP14 and Fetal Death
Carcinoma, Hepatocellular Antibodies	>1 publications with CASP14 and Carcinoma, Hepatocellular
Nerve Degeneration Antibodies	>1 publications with CASP14 and Nerve Degeneration
Liver Neoplasms Antibodies	>1 publications with CASP14 and Liver Neoplasms

Organs/Tissues associated with anti-CASP14 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>28 publications with CASP14 and Skin
Thymus Antibodies	>1 publications with CASP14 and Thymus
Lymph Node Antibodies	>1 publications with CASP14 and Lymph Node