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Tryptophan Hydroxylase (TPH1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175006

Description: Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2 (TPH1, TPH2) detection. Tested with WB in Human, rat, rabbit. No cross reactivity with other proteins. Uniprot ID: P17752Background:… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Tryptophan Hydroxylase (TPH1), Monoclonal Antibody
Full Product Name
Anti-Tryptophan Hydroxylase antibody (monoclonal)
Product Gene Name
anti-TPH1 antibody
Product Synonym Gene Name
TPH2
Product Synonym Names
Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2; tryptophan hydroxylase 1; tryptophan hydroxylase 2; TPH; TPRH; TRPH; ADHD7; NTPH
Clonality
Monoclonal
Isotype
IgG3
Clone Number
Try-63
Host
Mouse
Species Reactivity
Human, Rat, Rabbit
Form/Format
Lyophilized
Purity/Purification
Ascites
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
53,394 Da
OMIM
191060
3D Structure
ModBase 3D Structure for P17752
Immunogen
Recombinant rabbit tryptophan hydroxylase.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TPH1 antibody
Western Blot (WB)

Western Blot (WB) of anti-TPH1 antibody

Anti-Tryptophan Hydroxylase antibody (monoclonal), MBS175006, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Mouse Anti-TPH1 Monoclonal Antibody – Cat# MBS175006

Immunohistochemistry (IHC) of anti-TPH1 antibody

Applications: Immunohistochemistry
Sample: Wistar rat brain
Detection: Fluorescence
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."
Mouse Anti-TPH1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175006

Other Notes

Small volumes of anti-TPH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPH1 purchase

MBS175006 is an antibody targeting TPH1. Also known as Anti-Tryptophan Hydroxylase antibody (monoclonal). Reactive in Human, Rat, Rabbit. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPH1. It may not necessarily be applicable to this product.

NCBI GI #
116242823
NCBI GeneID
7166
NCBI Accession #
P17752.4
UniProt Primary Accession #
P17752
UniProt Secondary Accession #
O95188; O95189; Q16736; Q3KPG8; D3DQX6
UniProt Related Accession #
P17752
NCBI Official Full Name
Tryptophan 5-hydroxylase 1
NCBI Official Synonym Full Names
tryptophan hydroxylase 1
NCBI Official Symbol
TPH1
NCBI Official Synonym Symbols
TPRH; TRPH
UniProt Protein Name
Tryptophan 5-hydroxylase 1
UniProt Synonym Protein Names
Tryptophan 5-monooxygenase 1
Protein Family
Tryptophan 5-hydroxylase
UniProt Gene Name
TPH1
UniProt Synonym Gene Names
TPH; TPRH; TRPH
UniProt Entry Name
TPH1_HUMAN
NCBI Protein Information
tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; tryptophan 5-monooxygenase 1; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)

NCBI Summary for TPH1

This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]
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UniProt Comments for TPH1

TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.14.16.4

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; aromatic amino acid family metabolic process; indolalkylamine biosynthetic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process
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Research Articles on TPH1

1. HTTLPR may mediate the risk for SMB through modulation of some temperamental traits.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPH1 antibody

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies  Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
 Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies  Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Serotonergic Synapse Pathway antibodies  Serotonergic Synapse Pathway Diagram
 Serotonin And Melatonin Biosynthesis Pathway antibodies  Serotonin And Melatonin Biosynthesis Pathway Diagram
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