Rabbit Anti-NFKBIB Monoclonal Antibody – Cat# MBS1750062

Western blot analysis of IKB beta expression in Jurkat cell lysate (MBS1750062).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKBIB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFKBIB

IKB Beta (NFKBIB) Antibody – Rabbit Monoclonal

Name: NFKBIB Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750062
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750062

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IKB beta (NFKBIB), Monoclonal Antibody

Full Product Name

Anti-IKB beta Rabbit Monoclonal Antibody

Product Gene Name

anti-NFKBIB antibody

Product Synonym Names

NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; NFKBIB; IKBB; TRIP9

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

37771 MW

OMIM

L40407 Genomic DNA

3D Structure

ModBase 3D Structure for Q15653

Immunogen

A synthesized peptide derived from human IKB beta

Subcellular Localization

Cytoplasm. Nucleus.

Tissue Specificity

Expressed in all tissues examined.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-NFKBIB antibody

Signal Transduction; Signaling Pathway; Nuclear Signaling; NFKB Pathway; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; Immunology; Innate Immunity; TLR Signaling

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-NFKBIB antibody

Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-NFKBIB antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-NFKBIB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFKBIB purchase

MBS1750062 is an antibody targeting NFKBIB. Also known as Anti-IKB beta Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFKBIB. It may not necessarily be applicable to this product.

NCBI GI #

340805857

NCBI GeneID

4793

NCBI Accession #

NP_001230045.1

UniProt Primary Accession #

Q15653

UniProt Secondary Accession #

Q96BJ7; A8K3F4

UniProt Related Accession #

Q15653

GenBank Nucleotide #

NM_001243116.1

NCBI Official Full Name

NF-kappa-B inhibitor beta isoform 2

NCBI Official Synonym Full Names

NFKB inhibitor beta

NCBI Official Symbol

NFKBIB

NCBI Official Synonym Symbols

IKBB; TRIP9

UniProt Protein Name

NF-kappa-B inhibitor beta

UniProt Synonym Protein Names

I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9

Protein Family

NF-kappa-B inhibitor

UniProt Gene Name

NFKBIB

UniProt Synonym Gene Names

IKBB; TRIP9; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta; TR-interacting protein 9; TRIP-9

NCBI Protein Information

NF-kappa-B inhibitor beta

NCBI Summary for NFKBIB

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

UniProt Comments for NFKBIB

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Research Articles on NFKBIB

1. our data establish the importance of a novel tumor suppressive IKBB gene in abrogating angiogenesis in NPC via the NF-kappaB signalling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NFKBIB antibody

Reference Product	PubMed Publications
NFKBIA antibody	>6 publications with NFKBIB and NFKBIA
RELA antibody	>4 publications with NFKBIB and RELA
NFKB1 antibody	>3 publications with NFKBIB and NFKB1
NFKB2 antibody	>2 publications with NFKBIB and NFKB2
IKBKB antibody	>2 publications with NFKBIB and IKBKB
IKBKG antibody	>1 publications with NFKBIB and IKBKG
CHUK antibody	>1 publications with NFKBIB and CHUK

Pathways associated with anti-NFKBIB antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of NF-kappaB In B Cells Pathway antibodies	Activation Of NF-kappaB In B Cells Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
BCR Signaling Pathway antibodies	BCR Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram

Diseases associated with anti-NFKBIB antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with NFKBIB and Inflammation
Lung Diseases Antibodies	>2 publications with NFKBIB and Lung Diseases
Neoplasms Antibodies	>2 publications with NFKBIB and Neoplasms
Myocardial Infarction Antibodies	>1 publications with NFKBIB and Myocardial Infarction
Neoplasm Invasiveness Antibodies	>1 publications with NFKBIB and Neoplasm Invasiveness
Breast Neoplasms Antibodies	>1 publications with NFKBIB and Breast Neoplasms
Heart Diseases Antibodies	>1 publications with NFKBIB and Heart Diseases
Atherosclerosis Antibodies	>1 publications with NFKBIB and Atherosclerosis
Fatty Liver Antibodies	>1 publications with NFKBIB and Fatty Liver
Necrosis Antibodies	>1 publications with NFKBIB and Necrosis

Organs/Tissues associated with anti-NFKBIB antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with NFKBIB and Blood
Lung Antibodies	>2 publications with NFKBIB and Lung
Heart Antibodies	>1 publications with NFKBIB and Heart
Uterus Antibodies	>1 publications with NFKBIB and Uterus
Liver Antibodies	>1 publications with NFKBIB and Liver