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CDC23 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750064

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cdc23/APC8, Monoclonal Antibody
Full Product Name
Anti-Cdc23/APC8 Rabbit Monoclonal Antibody
Product Gene Name
anti-CDC23 antibody
Product Synonym Names
Cell division cycle protein 23 homolog; Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8; CDC23; ANAPC8
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
68834 MW
OMIM
AB011472 mRNA
3D Structure
ModBase 3D Structure for Q9UJX2
Immunogen
A synthesized peptide derived from human Cdc23/APC8
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CDC23 antibody

Cell Biology; Cell Cycle; Cell Division; spindle; Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; apc/c; cyclins; cyclin b family; Cancer
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CDC23 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-CDC23 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:100

Western Blot (WB) of anti-CDC23 antibody

Western blot analysis of Cdc23/APC8 expression in (1)HepG2 cell lysate; (2)Jurkat cell lysate (MBS1750064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC23 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC23
Rabbit Anti-CDC23 Monoclonal Antibody – Cat# MBS1750064

Other Notes

Small volumes of anti-CDC23 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC23 purchase

MBS1750064 is an antibody targeting CDC23. Also known as Anti-Cdc23/APC8 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC23. It may not necessarily be applicable to this product.

NCBI GI #
118402596
NCBI GeneID
8697
NCBI Accession #
NP_004652.2
UniProt Primary Accession #
Q9UJX2
UniProt Secondary Accession #
O75433; Q53FN2; Q9BS73; A8K6E5; B4E3A2; B7WP05; D3DQB7
UniProt Related Accession #
Q9UJX2
GenBank Nucleotide #
NM_004661.3
NCBI Official Full Name
cell division cycle protein 23 homolog
NCBI Official Synonym Full Names
cell division cycle 23
NCBI Official Symbol
CDC23
NCBI Official Synonym Symbols
APC8; CUT23; ANAPC8
UniProt Protein Name
Cell division cycle protein 23 homolog
UniProt Synonym Protein Names
Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8
Protein Family
Anaphase-promoting complex
UniProt Gene Name
CDC23
UniProt Synonym Gene Names
ANAPC8; APC8
NCBI Protein Information
cell division cycle protein 23 homolog

NCBI Summary for CDC23

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
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Research Articles on CDC23

1. Results identified CDC23 as a miR-34c-regulated target that could be responsible for the miR-34c-induced cell cycle arrest.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC23 antibody

 Reference Product  PubMed Publications
 CDC16 antibody  >24 publications with CDC23 and CDC16

Pathways associated with anti-CDC23 antibody

 Products by Pathway  Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram

Diseases associated with anti-CDC23 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with CDC23 and Neoplasms
 Breast Neoplasms Antibodies  >2 publications with CDC23 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >2 publications with CDC23 and Ovarian Neoplasms
 Gonadal Disorders Antibodies  >2 publications with CDC23 and Gonadal Disorders
 Carcinoma, Hepatocellular Antibodies  >2 publications with CDC23 and Carcinoma, Hepatocellular
 Ovarian Diseases Antibodies  >2 publications with CDC23 and Ovarian Diseases
 Liver Neoplasms, Experimental Antibodies  >1 publications with CDC23 and Liver Neoplasms, Experimental
 Liver Diseases Antibodies  >1 publications with CDC23 and Liver Diseases
 Adenocarcinoma Antibodies  >1 publications with CDC23 and Adenocarcinoma
 Death Antibodies  >1 publications with CDC23 and Death

Organs/Tissues associated with anti-CDC23 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with CDC23 and Liver
 Bone Antibodies  >1 publications with CDC23 and Bone
 Thyroid Antibodies  >1 publications with CDC23 and Thyroid
 Lung Antibodies  >1 publications with CDC23 and Lung
 Kidney Antibodies  >1 publications with CDC23 and Kidney
 Blood Antibodies  >1 publications with CDC23 and Blood
 Spleen Antibodies  >1 publications with CDC23 and Spleen
 Vascular Antibodies  >1 publications with CDC23 and Vascular
 Brain Antibodies  >1 publications with CDC23 and Brain
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