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5T4 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750094

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a- dependent LRP6 internalization. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
5T4, Monoclonal Antibody
Full Product Name
Anti-5T4 Rabbit Monoclonal Antibody
Product Gene Name
anti-5T4 antibody
Product Synonym Gene Name
TPBG
Product Synonym Names
Trophoblast glycoprotein; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1; WAIF1; TPBG; 5T4
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
46032 MW
OMIM
Z29083 mRNA
3D Structure
ModBase 3D Structure for Q13641
Immunogen
A synthesized peptide derived from human 5T4
Subcellular Localization
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-5T4 antibody

Stem Cells; Lineage Markers; Ectoderm; Stem Cells; Endoderm; mesoderm; Cancer; Tumor Immunology; tumor-associated antigens; Developmental Biology; Lineage Specification
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-5T4 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-5T4 antibody
WB: 1:500-1:2000
IP: 1:50

Western Blot (WB) of anti-5T4 antibody

Western blot analysis of 5T4 expression in MCF-7 cell lysate (MBS1750094).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPBG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TPBG
Rabbit Anti-5T4 Monoclonal Antibody – Cat# MBS1750094

Other Notes

Small volumes of anti-5T4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 5T4 purchase

MBS1750094 is an antibody targeting 5T4. Also known as Anti-5T4 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for 5T4. It may not necessarily be applicable to this product.

NCBI GI #
262205665
NCBI GeneID
7162
NCBI Accession #
NP_001159864.1
UniProt Primary Accession #
Q13641
UniProt Secondary Accession #
A8K555
UniProt Related Accession #
Q13641
GenBank Nucleotide #
NM_001166392.1
NCBI Official Full Name
trophoblast glycoprotein
NCBI Official Synonym Full Names
trophoblast glycoprotein
NCBI Official Symbol
TPBG
NCBI Official Synonym Symbols
5T4; M6P1; 5T4AG; WAIF1
UniProt Protein Name
Trophoblast glycoprotein
UniProt Synonym Protein Names
5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1; WAIF1
Protein Family
Trophoblast glycoprotein
UniProt Gene Name
TPBG
UniProt Synonym Gene Names
5T4; 5T4 oncotrophoblast glycoprotein; WAIF1
NCBI Protein Information
trophoblast glycoprotein

NCBI Summary for 5T4

This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]
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UniProt Comments for 5T4

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Research Articles on 5T4

1. We obtained GWS evidence (P < 2.7 x 10(-6)) for gene-based association in the total sample with a novel locus, TPBG (P = 1.8 x 10(-6)).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-5T4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with 5T4 and Neoplasms
 Disease Models, Animal Antibodies  >3 publications with 5T4 and Disease Models, Animal
 Lung Neoplasms Antibodies  >1 publications with 5T4 and Lung Neoplasms
 Kidney Diseases Antibodies  >1 publications with 5T4 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >1 publications with 5T4 and Neoplasms, Experimental

Organs/Tissues associated with anti-5T4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with 5T4 and Brain
 Embryonic Tissue Antibodies  >2 publications with 5T4 and Embryonic Tissue
 Kidney Antibodies  >1 publications with 5T4 and Kidney
 Intestine Antibodies  >1 publications with 5T4 and Intestine
 Eye Antibodies  >1 publications with 5T4 and Eye
 Lung Antibodies  >1 publications with 5T4 and Lung
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